Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
J Am Chem Soc. 2011 Nov 16;133(45):18312-7. doi: 10.1021/ja206983z. Epub 2011 Oct 26.
The duplex formation mediated by Hg(2+) in a properly designed ssDNA generates a stable hairpin structure, which greatly alters the translocation profile of the ssDNA through α-hemolysin nanopore. From the 2D-events contour plot, the presence of Hg(2+) can be confirmed in as little as 30 min at ∼7 nM or higher. The sensor is highly selective to Hg(2+), without interference from other metal ions. It can be fabricated from readily available materials, without the processes of synthesis, purification, probe-making, and so forth. This sensing strategy opens new possibilities for detecting many types of analytes which have specific interactions with DNA molecules.
Hg(2+)介导的双链体形成在经过适当设计的 ssDNA 中产生了一种稳定的发夹结构，这极大地改变了 ssDNA 通过α-溶血素纳米孔的迁移特性。从 2D 事件等高线图中可以确认，在大约 7 nM 或更高的浓度下，在 30 分钟内即可存在 Hg(2+)。该传感器对 Hg(2+)具有高度选择性，不受其他金属离子的干扰。它可以由现成的材料制成，而无需进行合成、纯化、探针制作等过程。这种传感策略为检测与 DNA 分子具有特异性相互作用的多种类型的分析物开辟了新的可能性。
