Mensah-Attipoe J, Reponen T, Salmela A, Veijalainen A-M, Pasanen P
Department of Environmental Science, University of Eastern Finland, Kuopio Campus, Kuopio, Finland.
Indoor Air. 2015 Jun;25(3):273-84. doi: 10.1111/ina.12140. Epub 2014 Jul 31.
Green building materials are becoming more popular. However, little is known about their ability to support or limit microbial growth. The growth of fungi was evaluated on five building materials. Two green, two conventional building materials and wood as a positive control were selected. The materials were inoculated with Aspergillus versicolor, Cladosporium cladosporioides and Penicillium brevicompactum, in the absence and presence of house dust. Microbial growth was assessed at four different time points by cultivation and determining fungal biomass using the N-acetylhexosaminidase (NAHA) enzyme assay. No clear differences were seen between green and conventional building materials in their susceptibility to support microbial growth. The presence of dust, an external source of nutrients, promoted growth of all the fungal species similarly on green and conventional materials. The results also showed a correlation coefficient ranging from 0.81 to 0.88 between NAHA activity and culturable counts. The results suggest that the growth of microbes on a material surface depends on the availability of organic matter rather than the classification of the material as green or conventional. NAHA activity and culturability correlated well indicating that the two methods used in the experiments gave similar trends for the growth of fungi on material surfaces.
绿色建筑材料正变得越来越受欢迎。然而，对于它们支持或限制微生物生长的能力却知之甚少。对五种建筑材料上真菌的生长情况进行了评估。选择了两种绿色建筑材料、两种传统建筑材料以及作为阳性对照的木材。在有无室内灰尘的情况下，将材料接种杂色曲霉、枝孢霉和短密青霉。通过培养并使用N - 乙酰己糖胺酶（NAHA）酶法测定真菌生物量，在四个不同时间点评估微生物生长情况。在支持微生物生长的易感性方面，绿色建筑材料和传统建筑材料之间未观察到明显差异。灰尘作为外部营养源的存在，同样促进了绿色和传统材料上所有真菌种类的生长。结果还显示，NAHA活性与可培养数量之间的相关系数在0.81至0.88之间。结果表明，微生物在材料表面的生长取决于有机物质的可利用性，而非材料是绿色还是传统的分类。NAHA活性与可培养性相关性良好，表明实验中使用的两种方法对于材料表面真菌生长呈现出相似的趋势。
