Coombs Kanistha, Vesper Stephen, Green Brett J, Yermakov Mikhail, Reponen Tiina
Department of Environmental Health, University of Cincinnati, P.O. Box 670056, Cincinnati, OH 45267-0056.
United States Environmental Protection Agency, 26 W. M. L. King Drive, Mail Stop 314, Cincinnati, OH 45268.
Int Biodeterior Biodegradation. 2017;125(0):251-257. doi: 10.1016/j.ibiod.2017.07.018.
Water-damaged buildings can lead to fungal growth and occupant health problems. Green building materials, derived from renewable sources, are increasingly utilized in construction and renovations. However, the question as to what fungi will grow on these green compared to non-green materials, after they get wet, has not been adequately studied. By determining what fungi grow on each type of material, the potential health risks can be more adequately assessed. In this study, we inoculated green and non-green pieces of ceiling tile, composite board, drywall, and flooring with indoor dust containing a complex mixture of naturally occurring fungi. The materials were saturated with water and incubated for two months in a controlled environment. The resulting fungal microbiomes were evaluated using ITS amplicon sequencing. Overall, the richness and diversity of the mycobiomes on each pair of green and non-green pieces were not significantly different. However, different genera dominated on each type of material. For example, spp. had the highest relative abundance on green and non-green ceiling tiles and green composite boards, but spp. dominated the non-green composite board. In contrast, spp. dominated green and non-green flooring samples. Green gypsum board was dominated by spp. and spp., but non-green gypsum board by spp. These data suggest that water-damaged green and non-green building materials can result in mycobiomes that are dominated by fungal genera whose member species pose different potentials for health risks.
受水损坏的建筑物会导致真菌生长和居住者健康问题。源自可再生资源的绿色建筑材料在建筑和翻新中越来越多地被使用。然而，与非绿色材料相比，这些绿色材料受潮后会生长何种真菌的问题尚未得到充分研究。通过确定每种材料上生长的真菌，可以更充分地评估潜在的健康风险。在本研究中，我们用含有天然存在的真菌复杂混合物的室内灰尘接种绿色和非绿色的天花板瓷砖、复合板、干墙和地板样本。将材料用水饱和，并在受控环境中培养两个月。使用ITS扩增子测序对产生的真菌微生物群落进行评估。总体而言，每对绿色和非绿色样本上真菌群落的丰富度和多样性没有显著差异。然而，每种材料上占主导地位的属不同。例如，某属在绿色和非绿色天花板瓷砖以及绿色复合板上相对丰度最高，但另一属在非绿色复合板上占主导地位。相比之下，又一属在绿色和非绿色地板样本中占主导地位。绿色石膏板由某两属主导，而非绿色石膏板由另一属主导。这些数据表明，受水损坏的绿色和非绿色建筑材料会导致真菌群落，这些群落由其成员物种对健康风险具有不同潜在影响的真菌属主导。
