Raman Research Institute, C.V. Raman Avenue, Sadashivanagar, Bangalore 560080, India; National Centre for Biological Sciences (TIFR), Bellary Road, Bangalore 560065, India.
National Centre for Biological Sciences (TIFR), Bellary Road, Bangalore 560065, India; Institute for Stem Cell Biology and Regenerative Medicine, Bellary Road, Bangalore 560065, India.
Curr Opin Cell Biol. 2014 Aug;29:126-32. doi: 10.1016/j.ceb.2014.05.007. Epub 2014 Jun 27.
A search for organizing principles underlying molecular patterning at the cell surface and its regulation over different scales is necessary. This is important for understanding how the cell builds membrane bound organelles that emanate from it and for how the cell interacts with its physical and chemical milieu. This requires a broad framework to rationalize the mass of accumulated data about the spatial localization and dynamics of its constituents, and their physical and chemical environment. Lateral heterogeneities in the organization of membrane components of a living cell appear to be a hallmark of how a cell addresses sorting and signaling functions. Here we explore two classes of mechanisms of segregation of membrane components in the plasma membrane. We suggest that viewing the membrane as a passive, thermally equilibrated system is unlikely to provide an adequate framework to understand the mechanisms of membrane component segregation in vivo. Instead the surface of living cells behaves as an active membrane composite.
有必要寻找细胞表面分子模式形成及其在不同尺度上调控的基本原理。这对于理解细胞如何构建从自身发出的膜结合细胞器以及细胞如何与其物理和化学环境相互作用非常重要。这需要一个广泛的框架来合理化关于其组成部分的空间定位和动力学及其物理和化学环境的大量累积数据。活细胞的膜成分的组织中的横向异质性似乎是细胞解决分选和信号转导功能的标志。在这里，我们探索了两种膜成分在质膜中隔离的机制。我们认为，将膜视为一个被动的、热平衡系统不太可能为理解体内膜成分隔离的机制提供一个充分的框架。相反，活细胞的表面表现为一个主动的膜复合材料。
