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南美洲和南极洲三种南丽蚌属物种(笠形腹足纲:丽蚌科)的新一代转录组特征分析

Next-generation transcriptome characterization in three Nacella species (Patellogastropoda: Nacellidae) from South America and Antarctica.

作者信息

Fuenzalida Gonzalo, Poulin Elie, Gonzalez-Wevar Claudio, Molina Cristian, Cardenas Leyla

机构信息

UPMC Univ Paris 06, UMR 7144, Equipe ABICE, Station Biologique de Roscoff, 29680 Roscoff, France; CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France.

Laboratorio de Ecología Molecular, Instituto de Ecología y Biodiversidad (IEB), Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras, 3425 Santiago, Chile.

出版信息

Mar Genomics. 2014 Dec;18 Pt B:89-91. doi: 10.1016/j.margen.2014.06.004. Epub 2014 Jun 27.

DOI:10.1016/j.margen.2014.06.004
PMID:24975986
Abstract

The southern tip of South America and Antarctica are particularly interesting due to many genera and also species currently sharing between both areas. The genus Nacella (Patellogastropoda: Nacellidae) is distributed in different regions of South America and Antarctica living preferentially on rocks and boulders and grazing on algae, diatoms and bacterial films. We described the transcriptomes of three Nacella species, Nacella concinna (Strebel, 1908), inhabiting the Antarctic Peninsula; Nacella magallanica (Gmelin, 1791), from Patagonia and Nacella clypeater (Lesson, 1831), from central Chile. In total, we obtained over 20,000 contigs with an average length of 583bp. Homologous protein coding genes (PCGs) for mitochondrial genome of the three species were characterized and a database of molecular markers was also generated. This study represents the first publicly available report on pyrosequencing data for patellogastropod species, and provides an important comparative resource for studies in ecophysiology and evolutionary adaptation in marine invertebrate species.

摘要

南美洲南端和南极洲尤其引人关注,因为目前这两个地区有许多属甚至物种是共有的。纳氏帽贝属(笠形腹足纲:纳氏帽贝科)分布于南美洲和南极洲的不同区域,主要生活在岩石和巨石上,以藻类、硅藻和细菌膜为食。我们描述了三种纳氏帽贝的转录组,分别是栖息在南极半岛的康氏纳氏帽贝(斯特雷贝尔,1908年)、来自巴塔哥尼亚的麦哲伦纳氏帽贝(格梅林,1791年)以及来自智利中部的圆盾纳氏帽贝(莱松,1831年)。我们总共获得了超过20000个重叠群,平均长度为583碱基对。对这三个物种线粒体基因组的同源蛋白质编码基因(PCGs)进行了表征,并建立了一个分子标记数据库。这项研究是关于笠形腹足纲物种焦磷酸测序数据的首份公开报告,为海洋无脊椎动物物种的生态生理学和进化适应性研究提供了重要的比较资源。

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