Fondevila D, Ferrer L, Ramos J A, Montane V, Ramis A J
Zentralbl Veterinarmed A. 1989 Jan;36(1):71-7. doi: 10.1111/j.1439-0442.1989.tb00704.x.
The present study concerns the immunocytochemical localization of S-100 protein and lysozyme in the cells of canine lymph nodes and lymphomas. In the normal canine lymph node S-100 protein was detected in follicular dendritic cells (FDCs) and in interdigitating reticulum cells (IRCs). S-100 positive cells were found in thirteen out of twenty-four lymphomas, scattered throughout the tumour or in follicles. In three cases (two cutaneous lymphomas and one nodal lymphoma) the number of positive cells was high, although the main proliferating cell population was S-100 negative. In normal lymph nodes lysozyme immunoreactivity was found in the cytoplasm of cortical and medullary histiocytes. Lymphomatous cells were negative for lysozyme, although in three cases lysozyme-positive cells were present between the lymphatic cells. The possible origin of these S-100 or lysozyme positive cells is discussed. It is suggested that S-100 protein and lysozyme can be used as useful markers for these two types of dendritic cells and for macrophages.
本研究关注犬淋巴结和淋巴瘤细胞中S-100蛋白和溶菌酶的免疫细胞化学定位。在正常犬淋巴结中，在滤泡树突状细胞（FDC）和交错突网状细胞（IRC）中检测到S-100蛋白。在24个淋巴瘤中的13个中发现了S-100阳性细胞，散布于整个肿瘤或滤泡中。在3例（2例皮肤淋巴瘤和1例淋巴结淋巴瘤）中，尽管主要增殖细胞群为S-100阴性，但阳性细胞数量较多。在正常淋巴结中，在皮质和髓质组织细胞的细胞质中发现溶菌酶免疫反应性。淋巴瘤细胞对溶菌酶呈阴性，尽管在3例中淋巴细胞之间存在溶菌酶阳性细胞。讨论了这些S-100或溶菌酶阳性细胞的可能来源。有人提出，S-100蛋白和溶菌酶可作为这两种树突状细胞和巨噬细胞的有用标志物。
