The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
The Advanced Technology Center, Sheba Medical Center, Ramat-Gan 52621, Israel.
Bioelectrochemistry. 2014 Oct;99:30-9. doi: 10.1016/j.bioelechem.2014.06.001. Epub 2014 Jun 17.
In spite of aggressive therapy, existing treatments offer poor prognosis for glioblastoma multiforme due to tumor infiltration into the surrounding brain as well as poor blood-brain barrier penetration of most therapeutic agents. In this paper we present a novel approach for a minimally invasive treatment and a non-invasive response assessment methodology consisting of applying intracranial point-source electroporation and assessing treatment effect volumes using magnetic resonance imaging. Using a unique setup of a single intracranial electrode and an external surface electrode we treated rats' brains with various electroporation protocols and applied magnetic resonance imaging to study the dependence of the physiological effects on electroporation treatment parameters. The extent of blood-brain barrier disruption and later volumes of permanent brain tissue damage were found to correlate significantly with the treatment voltages (r(2)=0.99, p<0.001) and the number of treatment pulses (r(2)=0.94, p<0.002). Blood-brain barrier disruption depicted 3.2±0.3 times larger volumes than the final permanent damage volumes (p<0.0001). These results indicate that it may be beneficial to use more than one modality of electroporation when planning a treatment for brain tumors.
尽管采用了积极的治疗方法，由于肿瘤浸润到周围的大脑以及大多数治疗药物难以穿透血脑屏障，胶质母细胞瘤的预后仍然很差。在本文中，我们提出了一种新的微创治疗方法和一种非侵入性的反应评估方法，包括颅内点源电穿孔和使用磁共振成像评估治疗效果体积。我们使用单个颅内电极和外部表面电极的独特设置，用各种电穿孔方案治疗大鼠的大脑，并应用磁共振成像研究生理效应与电穿孔治疗参数的关系。结果发现，血脑屏障破坏的程度和永久性脑组织损伤的体积与治疗电压（r²=0.99，p<0.001）和治疗脉冲数（r²=0.94，p<0.002）显著相关。血脑屏障破坏的体积比最终的永久性损伤体积大 3.2±0.3 倍（p<0.0001）。这些结果表明，在计划脑肿瘤治疗时，使用多种电穿孔模式可能会有益。
