Abeywardana Tharindumala, Pratt Matthew R
Department of Chemistry, University of Southern California, 840 Downey Way, Los Angeles, CA 90089 (USA).
Chembiochem. 2014 Jul 21;15(11):1547-54. doi: 10.1002/cbic.201402117. Epub 2014 Jun 27.
Chemistry has long played an indispensable role in biological discovery through the synthesis of homogeneous, structurally defined material. With continuing advances in the area of synthetic protein chemistry, chemists are able to prepare increasingly large and complex proteins that have enabled key biochemical experiments. Here, we describe some of the chemical methods that have been applied to the synthesis of ubiquitylated proteins, as ubiquitylation is a crucial post-translational modification that mediates a variety of important biological effects on substrate proteins.
长期以来，化学通过合成均相、结构明确的材料，在生物学发现中发挥了不可或缺的作用。随着合成蛋白质化学领域的不断进步，化学家能够制备出越来越大且复杂的蛋白质，这些蛋白质推动了关键的生物化学实验。在此，我们描述了一些已应用于泛素化蛋白质合成的化学方法，因为泛素化是一种关键的翻译后修饰，可介导对底物蛋白质的多种重要生物学效应。
