Virology Unit, Department of Translational Medicine, Novara Medical School, via Solaroli 17, 28100, Novara, Italy.
Br J Dermatol. 2014 Dec;171(6):1525-8. doi: 10.1111/bjd.13215. Epub 2014 Oct 26.
Research demonstrates an increased incidence of skin cancer in immunocompromised hosts, including patients with chronic lymphocytic leukaemia (CLL) and organ transplant recipients (OTRs). Active human β-papillomavirus (β-HPV) infection has been found in OTR skin lesions, suggesting its possible involvement in skin carcinogenesis. Merkel cell polyomavirus (MCPyV) has also been reported in cases of skin cancer.
To investigate the potential correlations between patient clinical features and skin cancer development, and the presence of β-HPV and MCPyV DNA and protein markers in skin lesions and hair bulbs from patients with CLL.
The clinical features of 293 patients with CLL were analysed according to the presence or absence of skin lesions. β-HPV and MCPyV infection was investigated in skin lesions and hair bulbs from the study cohort by both polymerase chain reaction (PCR) analysis and immunohistochemical screening.
No significant correlations were observed between any of the analysed haematological parameters and the development of skin cancer. PCR analysis revealed the presence of β-HPV and MCPyV DNA in skin lesions, and 83% of positivity for MCPyV DNA in hair bulbs, while systematic immunohistochemical analysis of all the lesions failed to detect any expression of the viral proteins β-HPV E4, L1 or MCPyV LTAg.
Overall, the data indicate that carriage of β-HPV and MCPyV in the lesional skin and hair bulbs from patients with CLL without any evident reactivation at skin tumour sites most likely represents coincidental rather than causal infection. This contrasts with previous findings in relation to OTR-derived skin lesions.
研究表明，包括慢性淋巴细胞白血病（CLL）患者和器官移植受者（OTR）在内的免疫功能低下宿主皮肤癌的发病率增加。在 OTR 皮肤病变中发现了活跃的人 β-乳头瘤病毒（β-HPV）感染，这表明其可能参与皮肤癌变。Merkel 细胞多瘤病毒（MCPyV）也已在皮肤癌病例中报道。
研究患者临床特征与皮肤癌发展之间的潜在相关性，以及 CLL 患者皮肤病变和毛囊中 β-HPV 和 MCPyV DNA 和蛋白标志物的存在。
根据皮肤病变的存在与否，分析了 293 例 CLL 患者的临床特征。通过聚合酶链反应（PCR）分析和免疫组织化学筛选，对研究队列的皮肤病变和毛囊中的 β-HPV 和 MCPyV 感染进行了研究。
未观察到任何分析的血液学参数与皮肤癌的发展之间存在显著相关性。PCR 分析显示皮肤病变中存在 β-HPV 和 MCPyV DNA，而毛囊中 MCPyV DNA 的阳性率为 83%，而对所有病变进行系统的免疫组织化学分析未能检测到任何病毒蛋白 β-HPV E4、L1 或 MCPyV LTAg 的表达。
总体而言，数据表明，CLL 患者皮肤病变和无明显皮肤肿瘤部位再激活的毛囊中携带 β-HPV 和 MCPyV 最可能代表偶然感染，而非因果感染。这与之前关于 OTR 衍生皮肤病变的发现形成对比。
