Ye Qing, Xu Xiao-Jun, Shao Wen-Xia, Pan Yan-Xiang, Chen Xue-Jun
Clinical Laboratory, The Children's Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
The Department of Hematology/Oncology, The Children's Hospital of Zhejiang University School of Medicine, Hangzhou 310003, China.
ScientificWorldJournal. 2014;2014:986527. doi: 10.1155/2014/986527. Epub 2014 Apr 7.
Mycoplasma pneumoniae (MP) infection is the dominant cause of pneumonia in children. We sought to determine the relationship between MP infection and secondary allergic disease and to clarify the associated mechanisms of inflammatory response. A prospective study was performed among 1330 patients diagnosed with pneumonia to investigate the patient immune status by determining the correlation between MP infection, immunoglobulin E (IgE) levels, and a spectrum of associated serum cytokines. Serum IgE, IL-4, IL-6, and IL-10 levels for MPP patients in the acute phase were obviously higher than those in the recovery phase (P < 0.01). MPP patients with allergic conditions had increased serum IgE levels and increased IL-4/INF- γ ratio, and IgE and Eosinophil Cationic Protein were further elevated in patients who eventually developed secondary asthma changes. Patients with severe pneumonia and high clinical pulmonary infection scores presented higher levels of IL-4 and IL-5 in serum than those with low scores (P < 0.01). The proportion of CD4+ and CD8+ T cells that secreted IL-4 was significantly increased in MPP patients with elevated IgE. Our data demonstrate a significant correlation between MP infection and IgE levels, which is associated with a Th1/Th2 cytokine imbalance.
肺炎支原体（MP）感染是儿童肺炎的主要病因。我们试图确定MP感染与继发性过敏性疾病之间的关系，并阐明炎症反应的相关机制。对1330例诊断为肺炎的患者进行了一项前瞻性研究，通过确定MP感染、免疫球蛋白E（IgE）水平和一系列相关血清细胞因子之间的相关性来调查患者的免疫状态。MPP患者急性期血清IgE、IL-4、IL-6和IL-10水平明显高于恢复期（P<0.01）。有过敏情况的MPP患者血清IgE水平升高，IL-4/INF-γ比值增加，最终出现继发性哮喘改变的患者IgE和嗜酸性粒细胞阳离子蛋白进一步升高。重症肺炎且临床肺部感染评分高的患者血清中IL-4和IL-5水平高于评分低的患者（P<0.01）。IgE升高的MPP患者中分泌IL-4的CD4+和CD8+T细胞比例显著增加。我们的数据表明MP感染与IgE水平之间存在显著相关性，这与Th1/Th2细胞因子失衡有关。
