Quesada Rita, Burdío Fernando, Iglesias Mar, Dorcaratto Dimitri, Cáceres Marta, Andaluz Anna, Poves Ignasi, Castiella Tomás, Sánchez Patricia, Berjano Enrique, Grande Luis
From the *Instituto Municipal de Investigación Médica; Departments of †Surgery and ‡Pathology, Hospital del Mar; and §Department of Animal Surgery, Veterinary Faculty, University of Barcelona, Barcelona; ∥Department of Pathology, Hospital Clínico Universitario "Lozano Blesa," Zaragoza; and ¶Department of Electronic Engineering, Polytechnic University of Valencia, Valencia, Spain.
Pancreas. 2014 Aug;43(6):931-7. doi: 10.1097/MPA.0000000000000156.
The aim of this study was to determine whether radiofrequency ablation (RFA) of the pancreas and subsequent transection of the main pancreatic duct may avoid the risk of both necrotizing pancreatitis and postoperative pancreatic fistula (POPF) formation.
Thirty-two rats were subjected to RFA and section of the pancreas over their portal vein. Animals were killed at 3, 7, 15, and 21 days (groups 0-3, respectively). Two additional control groups (sham operation and user manipulation only, respectively) of 15 days of postoperative period were considered. Postoperative complications, histological changes (including morphometric and immunohistochemical analysis), and incidence of POPF were evaluated.
A significant increase in serum amylase levels (P < 0.05) on the third postoperative day, which return to baseline levels in the following weeks, was noted in groups 0 to 3. Those groups showed a rapid atrophy of the distal pancreas by apoptosis with no signs of necrotizing pancreatitis or POPF. The distal pancreas in groups 1 to 3 compared with group 0 and control groups showed a significant increase of small islets (<1000 µm).
The rapid acinar atrophy of the distal pancreas after RFA and section of the pancreatic ducts in this model does not lead to necrotizing pancreatitis.
本研究旨在确定胰腺射频消融（RFA）及随后的主胰管横断是否可避免坏死性胰腺炎和术后胰瘘（POPF）形成的风险。
32只大鼠接受RFA并在门静脉上方横断胰腺。分别在术后3、7、15和21天处死动物（分别为0 - 3组）。另外考虑两个术后15天的对照组（分别为假手术组和仅进行操作组）。评估术后并发症、组织学变化（包括形态计量学和免疫组织化学分析）以及POPF的发生率。
0至3组术后第3天血清淀粉酶水平显著升高（P < 0.05），在接下来的几周内恢复至基线水平。这些组显示远端胰腺通过凋亡迅速萎缩，无坏死性胰腺炎或POPF的迹象。与0组和对照组相比，1至3组的远端胰腺中小胰岛（<1000 µm）显著增多。
在此模型中，RFA和胰管横断后远端胰腺的腺泡迅速萎缩不会导致坏死性胰腺炎。
