Schnell M, Perez-Roldan M J, Carney P S, Hillenbrand R
Opt Express. 2014 Jun 16;22(12):15267-76. doi: 10.1364/OE.22.015267.
We demonstrate quantitative phase mapping in confocal optical microscopy by applying synthetic optical holography (SOH), a recently introduced method for technically simple and fast phase imaging in scanning optical microscopy. SOH is implemented in a confocal microscope by simply adding a linearly moving reference mirror to the microscope setup, which generates a synthetic reference wave analogous to the plane reference wave of wide-field off-axis holography. We demonstrate that SOH confocal microscopy allows for non-contact surface profiling with sub-nanometer depth resolution. As an application for biological imaging, we apply SOH confocal microscopy to map the surface profile of an onion cell, revealing nanoscale-height features on the cell surface.
我们通过应用合成光学全息术（SOH）在共聚焦光学显微镜中实现了定量相位映射，SOH是一种最近引入的用于扫描光学显微镜中技术简单且快速的相位成像方法。通过在显微镜设置中简单地添加一个线性移动的参考镜，SOH在共聚焦显微镜中得以实现，该参考镜产生一个类似于宽场离轴全息术的平面参考波的合成参考波。我们证明，SOH共聚焦显微镜能够实现具有亚纳米深度分辨率的非接触表面轮廓分析。作为生物成像的应用，我们将SOH共聚焦显微镜应用于绘制洋葱细胞的表面轮廓，揭示了细胞表面的纳米级高度特征。
