Schnell Martin, King Melanie, Buercklin Sam, Sarriugarte Paulo, Hillenbrand Rainer, Carney P Scott
Opt Lett. 2023 Sep 1;48(17):4424-4427. doi: 10.1364/OL.496111.
We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. In this physics-based approach, computational propagation is implemented on the complex signal recovered in synthetic holography, consistent with wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation, with a potential application in confocal optical surface profiling.
我们展示了基于合成光学全息术的相干共聚焦激光扫描显微镜中的数值重聚焦。在这种基于物理的方法中，计算传播是在合成全息术中恢复的复信号上实现的，这与波动物理学和显微镜的参数一致。实验证明，从在几个焦平面偏移处采集的数据中恢复了测试物体的聚焦图像。数值重聚焦可以为高度变化较大的样品提供聚焦视图，在共焦光学表面轮廓测量中有潜在应用。
