利用光学微环谐振器检测生物素衍生化聚烯丙胺上的蛋白质。
Protein detection on biotin-derivatized polyallylamine by optical microring resonators.
作者信息
Ullien Daniela, Harmsma Peter J, Abdulla Shahina M C, de Boer Bart M, Bosma Duco, Sudhölter Ernst J R, de Smet Louis C P M, Jager Wolter F
出版信息
Opt Express. 2014 Jun 30;22(13):16585-94. doi: 10.1364/OE.22.016585.
Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies via a streptavidin-biotin binding scheme. Monitoring the shift in the microring resonance wavelength allows real-time, highly sensitive detection of the biomolecular interaction.
硅光学微环谐振器（MRR）是可用于生物传感的敏感器件。我们基于在这类MRR上应用聚电解质（PE）层，提出了一种新型生物传感平台。顶部的PE层用生物素进行了共价标记，以通过链霉亲和素-生物素结合方案确保抗体的结合位点。监测微环谐振波长的变化可实现对生物分子相互作用的实时、高灵敏度检测。
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