Livny Eitan, Parker Jack S, van der Kaaij Mariëlle, Haasdijk Elize D, van der Wees Jacqueline, Bruinsma Marieke, Melles Gerrit R J
*Melles Cornea Clinic Rotterdam, Rotterdam, the Netherlands; †Netherlands Institute for Innovative Ocular Surgery, Rotterdam, the Netherlands; ‡Amnitrans Eye Bank Rotterdam, Rotterdam, the Netherlands; and §Department of Neuroscience, Erasmus Medical Center Rotterdam, the Netherlands.
Cornea. 2014 Aug;33(8):790-4. doi: 10.1097/ICO.0000000000000156.
The aim of this study was to describe the ultrastructure of the host-donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty.
The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation.
Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal "virgin" corneal architecture.
Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.
本研究旨在描述一名近期去世患者眼中供体与受体界面的超微结构，该患者接受了Descemet膜内皮角膜移植术。
在患者死后摘除眼球，经过标准消毒后，切除角膜巩膜纽扣，切成4个象限，进行光镜和透射电镜评估。
透射电镜显示供体的Descemet膜与受体基质紧密相连，基质胶原纤维向界面基质内突出，类似于正常的“原始”角膜结构。
在超微结构上，附着的Descemet膜内皮角膜移植片与未手术的健康眼睛非常相似，没有明显的外来或缺失结构。
