Charretier E, Wietzerbin J
Service de Biophysique, Département de Biologie, Gif-sur-Yvette, France.
Biochimie. 1989 Jan;71(1):67-70. doi: 10.1016/0300-9084(89)90133-8.
The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These results support the idea that both derivatives induce outward proton movement from the cell to the external solution. In this respect, the non-esterified derivative proved to be more powerful that the esterified one. Under the present conditions, there was little or no regulation of pH in HL-60 cells, which exhibited an almost constant pH gradient between the external and internal pH (acid inside relative to outside). This deficiency in pH homeostasis might be due to the immature state of the HL-60 cells.
在HL-60早幼粒细胞白血病细胞上测试了两性霉素B的两种多烯衍生物，N-果糖基两性霉素B和N-果糖基两性霉素B甲酯对细胞内和细胞外pH的影响。在弱缓冲培养基中，这两种衍生物均提高了细胞内pH并降低了细胞外pH。这些结果支持了这两种衍生物均诱导质子从细胞向外部溶液外流的观点。在这方面，未酯化的衍生物比酯化的衍生物更有效。在当前条件下，HL-60细胞几乎没有或没有pH调节，细胞内外pH之间呈现几乎恒定的pH梯度（相对于外部，内部呈酸性）。pH稳态的这种缺陷可能是由于HL-60细胞的不成熟状态所致。
