Newton G R, Hansen P J, Bazer F W, Leslie M V, Stephenson D C, Low B G
Department of Dairy, University of Florida, Gainesville 32611-0701.
Biol Reprod. 1989 Feb;40(2):417-24. doi: 10.1095/biolreprod40.2.417.
Allantoic and amniotic fluids were collected on Days 60 (n = 3), 100 (n = 4), and 140 (n = 3) of pregnancy. The presence of uterine milk proteins (UTM-proteins) in these samples was evaluated by Ouchterlony immunodiffusion and enzyme-linked immunoabsorbant assay (ELISA). Eight of ten samples of allantoic fluid and three of ten samples of amniotic fluid produced one or two immunoprecipitin bands against antiserum to UTM-proteins. Each band fused with immunoprecipitin bands from UTM-proteins purified from uterine fluid. Data from a semi-quantitative ELISA indicated that allantoic fluid from all ewes and amniotic fluid from six of ten ewes contained immunoreactive UTM-proteins. Concentrations of UTM-proteins in these fluids were not statistically affected by day of gestation (p greater than 0.10), but tended to decline as gestation advanced. Greater concentrations of UTM-proteins were detected in allantoic fluid than in amniotic fluid (p less than 0.05). The physical characteristics of the immunoreactive material in allantoic and amniotic fluids were examined by polyacrylamide gel electrophoresis and Western blotting. The immunoreactive material was found to possess pIs and molecular weights identical to UTM-proteins. These results indicate that fetal fluids contain material that reacts with antiserum to UTM-proteins and has physical properties similar to UTM-proteins. It is likely, therefore, that the UTM-proteins are transported across the placenta during gestation, perhaps to serve some function in the fetal compartment.
在妊娠第60天（n = 3）、100天（n = 4）和140天（n = 3）采集尿囊液和羊水。通过双向免疫扩散和酶联免疫吸附测定（ELISA）评估这些样本中子宫乳蛋白（UTM - 蛋白）的存在情况。十个尿囊液样本中的八个以及十个羊水样本中的三个，针对UTM - 蛋白抗血清产生了一条或两条免疫沉淀带。每条带都与从子宫液中纯化的UTM - 蛋白的免疫沉淀带融合。半定量ELISA的数据表明，所有母羊的尿囊液以及十分之六的母羊的羊水中都含有免疫反应性UTM - 蛋白。这些液体中UTM - 蛋白的浓度在统计学上不受妊娠天数的影响（p大于0.10），但随着妊娠进展有下降趋势。尿囊液中检测到的UTM - 蛋白浓度高于羊水（p小于0.05）。通过聚丙烯酰胺凝胶电泳和蛋白质免疫印迹法检测尿囊液和羊水中免疫反应性物质的物理特性。发现该免疫反应性物质的等电点和分子量与UTM - 蛋白相同。这些结果表明，胎儿体液中含有与UTM - 蛋白抗血清发生反应且物理性质与UTM - 蛋白相似的物质。因此，很可能UTM - 蛋白在妊娠期间通过胎盘转运，可能在胎儿体内发挥某种功能。
