Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory 7925 Cape Town, South Africa.
Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory 7925 Cape Town, South Africa; Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7925 Cape Town, South Africa.
Exp Eye Res. 2014 Aug;125:226-35. doi: 10.1016/j.exer.2014.06.011. Epub 2014 Jun 27.
The mouse eye has been used as a model for studies on the microanatomy of the outflow pathways but most of what is known comes from histological sections. These studies have focused mainly on the morphological features of the trabecular meshwork, Schlemm's canal and aqueous channels that link to the superficial episcleral vasculature. However, the anatomical architecture of the aqueous outflow vessels and their relationship to each other and to the general vascular circulation is not well understood. The aim of this study was to provide a detailed description of the microarchitecture of the aqueous outflow vessels and their relationship to the superficial limbal/episcleral vasculature throughout the entire limbus. The aqueous outflow vessels and blood and lymphatic vessels were imaged in PECAM-1 and LYVE-1 immunostained whole anterior segments of adult mice and three-dimensional (3-D) reconstructions of the optical sections were generated to reveal the aqueous, blood and lymphatic architecture. The arterial supply, venous drainage, organisation of perilimbal vasculature, collector channels/aqueous veins and the morphology of Schlemm's canal were revealed in their entirety and the relationships between these structures is described. Schlemm's canal was PECAM-1 positive but there was no affinity for the lymphatic marker LYVE-1. We show that Schlemm's canal is a continuous circular structure and more often seen as a single, broad, varicose vessel with short regions appearing as a plexus. Aqueous veins link Schlemm's canal to the superficial vasculature and there were no direct links seen between the canal and the lymphatic vessels.
小鼠眼睛一直被用作研究流出道微解剖结构的模型，但我们所了解的大部分内容都来自组织学切片。这些研究主要集中在小梁网、施莱姆氏管和与浅层巩膜脉管系统相连的房水通道的形态特征上。然而，房水流出血管的解剖结构及其相互之间以及与一般血管循环的关系尚不清楚。本研究旨在详细描述整个角膜缘的房水流出血管的微观结构及其与浅层角膜缘/巩膜脉管系统的关系。在成年小鼠的 PECAM-1 和 LYVE-1 免疫染色的整个前节中对房水流出血管以及血液和淋巴管进行成像，并生成光学切片的三维（3-D）重建，以揭示房水、血液和淋巴的结构。揭示了动脉供应、静脉引流、角膜缘血管组织、收集管/房水静脉以及施莱姆氏管的形态，描述了这些结构之间的关系。施莱姆氏管呈 PECAM-1 阳性，但对淋巴管标志物 LYVE-1 没有亲和力。我们表明，施莱姆氏管是一个连续的圆形结构，更常见的是单个宽阔的、静脉曲张的血管，短区域呈丛状。房水静脉将施莱姆氏管与浅层血管相连，在管和淋巴管之间没有直接联系。
