Grce Magdalena
Acta Med Croatica. 2013 Dec;67(5):425-32.
Many viruses, fungi and bacteria inhabit the mouth. Most of these microorganisms can be easily cultured and identified by classical microbiological tests, but some of them, such as human papilloma virus, can only be identified by the methods of molecular biology. Some microorganisms are present in a very small amount and cannot be detected by classical tests; therefore, in this case, molecular diagnosis is also a logical alternative. Molecular biology assays are sensitive, objective, easy to perform and less demanding than conventional microbiological methods in terms of training and quality assurance. This paper describes the molecular methods that are commonly used for the detection of microorganisms that colonize the oral cavity.
口腔中存在许多病毒、真菌和细菌。这些微生物大多可通过经典微生物学检测方法轻松培养和鉴定，但其中一些，如人乳头瘤病毒，只能通过分子生物学方法鉴定。一些微生物数量极少，无法通过经典检测方法检测到；因此，在这种情况下，分子诊断也是一种合理的选择。分子生物学检测方法灵敏、客观、易于操作，且在培训和质量保证方面比传统微生物学方法要求更低。本文介绍了常用于检测口腔定植微生物的分子方法。
