Morris A G, Ward G A, Bateman W J
Department of Biological Sciences, University of Warwick, Coventry, United Kingdom.
Cell Immunol. 1989 May;120(2):470-6. doi: 10.1016/0008-8749(89)90214-1.
C3H10T1/2 fibroblasts when transformed with Kirsten murine sarcoma virus lose the ability to be induced to express class II major histocompatibility complex antigens when induced with interferon-gamma (IFN-gamma). Sublines were derived from transformed lines by cell sorting after treatment with IFN-gamma, sorting for low or high expression of H-2Ak. These sublines remained stably noninducible or inducible for class II antigen for several passages after sorting. In all other respects tested, viz, sensitivity to IFN-gamma for the generation of an antiviral state or the induction of class I antigen, content of ras gene products, the sorted sublines were very similar. We conclude that ras oncogene expression in these cells can influence the induction of class II antigen but that because ras expression in the sorted lines is similar the effect of ras expression is indirect and presumably involves interaction with other cellular factors.
当用 Kirsten 鼠肉瘤病毒转化时，C3H10T1/2 成纤维细胞在用γ干扰素（IFN-γ）诱导时失去了被诱导表达 II 类主要组织相容性复合体抗原的能力。在用 IFN-γ处理后通过细胞分选从转化细胞系中获得亚系，分选 H-2Ak 的低表达或高表达。这些亚系在分选后经过几代传代仍保持稳定的不可诱导或可诱导 II 类抗原的状态。在测试的所有其他方面，即对 IFN-γ产生抗病毒状态或诱导 I 类抗原的敏感性、ras 基因产物的含量，分选后的亚系非常相似。我们得出结论，这些细胞中 ras 癌基因的表达可以影响 II 类抗原的诱导，但由于分选细胞系中 ras 的表达相似，ras 表达的影响是间接的，大概涉及与其他细胞因子的相互作用。
