小型化：基于芯片的液相色谱和蛋白质组学。
Miniaturization: Chip-based liquid chromatography and proteomics.
作者信息
Gauthier Georges L, Grimm Rudolf
机构信息
Agilent Technologies R&D and Marketing GmbH & Co. KG, 76337 Waldbronn, Germany.
Agilent Technologies, Integrated Biology Solutions Unit, 95052-8059 Santa Clara, USA.
出版信息
Drug Discov Today Technol. 2006 Spring;3(1):59-66. doi: 10.1016/j.ddtec.2006.03.013.
Proteomic research is linked with significant technological challenges such as the high dynamic range and low abundance of biologically interesting proteins. Moreover, there is an increasing need for high-throughput and robustness of routinely performed analyses. Solving these difficulties requires refinements in the capability to fractionate and prepare biological samples as well as improvements in speed, automation, separation power and overall analytical sensitivity.Recent innovations in microfluidic devices with integrated on-chip sample enrichment, liquid chromatography and electrospray emitters and their applicability for specific proteomic applications are presented in this review.:
蛋白质组学研究面临着重大的技术挑战，例如具有生物学意义的蛋白质动态范围高且丰度低。此外，对于常规分析的高通量和稳健性的需求也日益增加。解决这些难题需要在生物样品的分级分离和制备能力方面进行改进，同时还要提高速度、自动化程度、分离能力以及整体分析灵敏度。本综述介绍了具有集成芯片上样品富集、液相色谱和电喷雾发射器的微流控装置的最新创新及其在特定蛋白质组学应用中的适用性。
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