Usami Atsushi, Nakahashi Hiroshi, Marumoto Shinsuke, Miyazawa Mitsuo
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka-shi, Osaka, 577-8502, Japan.
Phytochem Anal. 2014 Nov-Dec;25(6):561-6. doi: 10.1002/pca.2528. Epub 2014 Jun 30.
The Chrysanthemum genus consisting of about 200 species is mainly distributed over the Northern Hemisphere. Despite the pleasant odour of C. japonense var. debile (setonojigiku), no detailed analysis of the aroma-active compounds has been reported using sensory evaluation.
Using a hydrodistillation (HD) and a solvent-assisted flavour evaporation (SAFE) method to obtain the volatile oil from the leaf parts.
To clarify odorants contributing to the characteristic aroma-active compounds, the aroma-extract dilution analysis (AEDA) method was performed through gas chromatography olfactometry (GC/O) analysis. In addition, the odour activity value (OAV) was calculated in order to determine the relative contribution of each compound to the aroma-active compounds.
A total of 42 components by HD oil were identified by GC-MS, whereas 34 components were identified in SAFE oil. Thirteen compounds were identified by GC/O analysis in HD and SAFE oils respectively.
Each extraction method has its own advantages and disadvantages, and they are generally complementary to each other. On the basis of AEDA, OAV and sensory evaluations, [2.2.1] bicyclic monoterpenes (borneol, bornyl acetate and camphor) and β-caryophyllene are considered to be the main aroma-active compounds of both extraction methods.
菊花属约有200个物种，主要分布在北半球。尽管弱小滨菊（C. japonense var. debile）（setonojigiku）气味宜人，但尚未有使用感官评价对其香气活性成分进行详细分析的报道。
采用水蒸馏（HD）和溶剂辅助风味蒸发（SAFE）方法从叶片部分获取挥发油。
为了阐明对特征香气活性化合物有贡献的气味物质，通过气相色谱 - 嗅觉测定法（GC/O）分析进行香气提取物稀释分析（AEDA）。此外，计算气味活性值（OAV）以确定每种化合物对香气活性化合物的相对贡献。
通过GC - MS鉴定出HD油中的42种成分，而SAFE油中鉴定出34种成分。分别在HD油和SAFE油中通过GC/O分析鉴定出13种化合物。
每种提取方法都有其优缺点，它们通常相互补充。基于AEDA、OAV和感官评价，[2.2.1]双环单萜（冰片、乙酸龙脑酯和樟脑）和β-石竹烯被认为是两种提取方法的主要香气活性化合物。
