Usami Atsushi, Ono Toshirou, Kashima Yusei, Nakahashi Hiroshi, Marumoto Shinsuke, Nosaka Sota, Watanabe Shogo, Miyazawa Mitsuo
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University.
J Oleo Sci. 2014;63(1):83-92. doi: 10.5650/jos.ess13043. Epub 2013 Dec 18.
The chemical composition of volatile oil from agitake (Pleurotus eryngii var. ferulae) was established for the first time using gas chromatography (GC) and GC-mass spectrometry. Sixty-seven and 24 components were extracted by hydrodistillation (HD) using diethyl ether (DE) and dichloromethane (DM), respectively; these components accounted for 80.3% and 91.8% of the total oil, respectively. Thirteen and 48 components of were extracted by the solvent-assisted flavor evaporation method (SAFE), using DE and DM, respectively, and identified; these components accounted for 83.5% and 82.0% of the total oil, respectively. Methylsuccinimide and 2,3,7-trimethyl-2-octene were the most characteristic components by SAFE using DM.Odor evaluation of the volatile oil from agitake was also carried out using GC-olfactometry (GC-O), aroma extraction dilution analysis (AEDA), and the odor activity value (OAV). Sixteen, 8, 5 and 9 aroma-active components were identified using HD (DE and DM) and SAFE (DE and DM), respectively. The main aroma-active components extracted using HD and SAFE were 1-octen-3-ol (mushroom-like) and phenylacetaldehyde (floral), respectively. This study proved that HD and SAFE can be used as complementary extraction techniques for the complete characterization of volatile oil from agitake.
首次采用气相色谱（GC）和GC-质谱联用技术确定了白灵侧耳挥发油的化学成分。分别使用乙醚（DE）和二氯甲烷（DM）通过水蒸馏法（HD）提取出67种和24种成分；这些成分分别占总油的80.3%和91.8%。分别使用DE和DM通过溶剂辅助风味蒸发法（SAFE）提取并鉴定出13种和48种成分；这些成分分别占总油的83.5%和82.0%。使用DM的SAFE法中，甲基琥珀酰亚胺和2,3,7-三甲基-2-辛烯是最具特征的成分。还使用气相色谱-嗅觉测量法（GC-O）、香气提取稀释分析（AEDA）和气味活性值（OAV）对白灵侧耳挥发油进行了气味评估。分别使用HD（DE和DM）和SAFE（DE和DM）鉴定出16种、8种、5种和9种香气活性成分。使用HD和SAFE提取的主要香气活性成分分别是1-辛烯-3-醇（蘑菇样）和苯乙醛（花香）。本研究证明，HD和SAFE可作为互补提取技术用于全面表征白灵侧耳挥发油。
