Popa-Burke Ioana, Russell John
Sample Management Technologies, Molecular Discovery Research, GlaxoSmithKline, Research Triangle Park, NC, USA
Sample Management Technologies, Molecular Discovery Research, GlaxoSmithKline, Research Triangle Park, NC, USA.
J Biomol Screen. 2014 Oct;19(9):1302-8. doi: 10.1177/1087057114541146. Epub 2014 Jun 30.
Screening compounds precipitate out of high-concentration DMSO solutions and cause issues in the liquid-handling equipment used to process the samples as well as false-positives and false-negatives in the biological data. This report examines the extent of the precipitation issue, correlation with physico-chemical properties of compounds, and measured concentrations. Possible ways to allow recovery of the precipitated solids are also described. This study also investigated whether particulates were present from the time of initial solubilization in DMSO or whether precipitation occurs during freeze-thaw cycling.
筛选化合物会从高浓度二甲基亚砜（DMSO）溶液中沉淀出来，从而在用于处理样品的液体处理设备中引发问题，并导致生物数据出现假阳性和假阴性。本报告研究了沉淀问题的严重程度、与化合物物理化学性质的相关性以及测量浓度。还描述了回收沉淀固体的可能方法。本研究还调查了从最初在DMSO中溶解时是否就存在颗粒，或者在冻融循环过程中是否会发生沉淀。
