Nenaah Gomah E
a Zoology Department , Faculty of Science, Kafrelsheikh University , 33516 Kafr El Sheikh , Egypt.
Nat Prod Res. 2014;28(24):2245-52. doi: 10.1080/14786419.2014.932788. Epub 2014 Jul 1.
Four prenylated flavonoids, isoglabratephrin, (+)-glabratephrin, tephroapollin-F and lanceolatin-A, were isolated from Tephrosia apollinea L. and tested against three stored grain insects. Using the filter paper bioassay, compounds showed adulticidal activity against Sitophilus oryzae (L), Rhyzopertha dominica (F) and Tribolium castaneum (Herbst) at concentrations of 0.875, 1.75 and 3.5 mg mL(- 1). At 3.5 mg mL(- 1), tephroapollin-F was the most toxic (78.6%, 64.6% and 60.7% mortality was recorded after 10 days exposure of S. oryzae, R. dominica and T. castaneum, respectively). The F1 progeny production of insects was affected after parental exposure to flavonoids, where S. oryzae was the most susceptible. A nutritional bioassay, employing a flour disc and test concentrations of 0.65, 1.3 and 2.6 mg g(- 1), revealed a significant reduction in the relative growth rate, relative consumption rate and efficiency of conversion of ingested food by all insects. The structure-activity relationship among the tested flavonoids was discussed.
从紫穗槐中分离出四种异戊烯基黄酮，即异毛鱼藤素、（+）-毛鱼藤素、灰毛豆素-F和披针叶鱼藤素-A，并对三种储粮害虫进行了测试。采用滤纸生物测定法，这些化合物在浓度为0.875、1.75和3.5 mg mL⁻¹时，对米象（L）、谷蠹（F）和赤拟谷盗（Herbst）表现出成虫icidal活性。在3.5 mg mL⁻¹时，灰毛豆素-F毒性最强（米象、谷蠹和赤拟谷盗分别暴露10天后，死亡率分别为78.6%、64.6%和60.7%）。亲代接触黄酮类化合物后，昆虫的F1后代产量受到影响，其中米象最为敏感。采用面粉圆盘和0.65、1.3和2.6 mg g⁻¹的测试浓度进行的营养生物测定表明，所有昆虫的相对生长率、相对消耗率和摄入食物转化率均显著降低。讨论了受试黄酮类化合物之间的构效关系。
