Zuikov S A, Borzenko B G, Shatova O P, Bakurova E M, Polunin G E
Department of Chemistry, Maksim Gorky National Medical University, Donetsk 83003, Ukraine.
Department of General Surgery and Surgical Diseases, Maksim Gorky National Medical University, Donetsk 83003, Ukraine.
Exp Oncol. 2014 Jun;36(2):117-20.
To examine the relationship between metabolic features of purine nucleotides and antioxidant system depending on the age of patients with colorectal cancer.
The activity of adenosine deaminase, xanthine oxidase, glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase, the NOx concentration and the oxidative modification of proteins were determined spectrophotometricaly in 50 apparently healthy people and 26 patients with colorectal cancer stage -III---IV, aged 40 to 79 years.
Increase of pro-oxidant system of erythrocytes with the age against decrease in level of antioxidant protection in both healthy individuals and colorectal cancer patients was determined. A significant increase of pro-ducts of oxidative proteins modification in erythrocytes with ageing was shown. Statistically significant correlation between enzymatic and non enzymatic markers pro-oxidant system and the activity of antioxidant defense enzymes in erythrocytes of patient with colorectal cancer was determined.
Obtained results have demonstrated the imbalance in the antioxidant system of erythrocytes in colorectal cancer patients that improve the survival of cancer cells that is more distinctly manifested in ageing.
根据结直肠癌患者的年龄，研究嘌呤核苷酸代谢特征与抗氧化系统之间的关系。
采用分光光度法测定了50名40至79岁的表观健康者以及26名III-IV期结直肠癌患者红细胞中腺苷脱氨酶、黄嘌呤氧化酶、谷胱甘肽过氧化物酶、超氧化物歧化酶和葡萄糖-6-磷酸脱氢酶的活性、NOx浓度以及蛋白质的氧化修饰情况。
在健康个体和结直肠癌患者中，均发现随着年龄增长红细胞促氧化系统增加，而抗氧化保护水平下降。结果显示，随着年龄增长红细胞中氧化蛋白质修饰产物显著增加。结直肠癌患者红细胞中促氧化系统的酶促和非酶促标志物与抗氧化防御酶活性之间存在统计学显著相关性。
研究结果表明，结直肠癌患者红细胞抗氧化系统失衡，这提高了癌细胞的存活率，且在衰老过程中表现得更为明显。
