Yang Fengyong, Liu Zhen, Wang Yajing, Li Zhaoxin, Yu Haichu, Wang Qixin
ICU, Affiliated Hospital of Qingdao University, 21 Jiangsu Road, Qingdao, 266000, China.
Cell Biochem Biophys. 2014 Dec;70(3):1683-6. doi: 10.1007/s12013-014-0113-3.
The aim of the study was to evaluate the clinical efficacy of hydrogen sulfide (H2S) treatment on the endothelin-induced cardiac hypertrophy. Sixty-four adult male rats, weighing from 180 to 200 g, were randomly divided into four groups: ten in normal group, ten in sham group, 44 in model group established by inducing the myocardial hypertrophy with endothelin. The myocardial hypertrophy model rats were randomly divided into two groups: 22 in the simple myocardial hypertrophy model group and 22 in the H2S treatment group. Rats in normal group were given 2 ml pure water by gavage per day, those in the sham group and simple cardiac hypertrophy model group were given 2 ml of saline by gavage per day, and rats in the pure cardiac hypertrophy with H2S treatment were given intraperitoneal injections of 2 ml NaHS saline per day for a period of 4 weeks. Left ventricular mass index, myocyte hypertrophy, volume fraction of myocardial interstitial collagen, myocardial hydroxyproline content and other indicators of cardiac hypertrophy were observed after 4 weeks. (1) There were significant differences on the ventricular mass between the treatment group and the cardiac hypertrophy group: The left ventricular mass decreased 21.4 % and the left ventricular mass index decreased 5.97 % (P < 0.05; (2) the smallest cardiomyocytes diameter and cardiomyocytes cross-sectional area decreased 12.5 and 10.8 %, respectively (P < 0.05) in the treatment group compared to the cardiac hypertrophy group; (3) the volume fraction of myocardial interstitial collagen and the myocardial hydroxyproline content decreased 22.3 and 31.3 % in treatment group compared with the cardiac hypertrophy group, respectively (P < 0.05). H2S had a good clinical efficacy in reducing left ventricular mass fraction and myocardial collagen levels, improving myocardial hypertrophy and decrease myocardial fibrosis. It is worthy for further clinical studies.
本研究旨在评估硫化氢（H₂S）治疗内皮素诱导的心肌肥大的临床疗效。将64只体重180至200克的成年雄性大鼠随机分为四组：正常组10只，假手术组10只，用内皮素诱导心肌肥大建立模型组44只。将心肌肥大模型大鼠随机分为两组：单纯心肌肥大模型组22只和H₂S治疗组22只。正常组大鼠每天经口灌胃给予2毫升纯水，假手术组和单纯心肌肥大模型组大鼠每天经口灌胃给予2毫升生理盐水，H₂S治疗的单纯心肌肥大组大鼠每天腹腔注射2毫升硫氢化钠生理盐水，持续4周。4周后观察左心室质量指数、心肌细胞肥大、心肌间质胶原容积分数、心肌羟脯氨酸含量等心肌肥大指标。（1）治疗组与心肌肥大组心室质量有显著差异：左心室质量下降21.4%，左心室质量指数下降5.97%（P<0.05）；（2）与心肌肥大组相比，治疗组最小心肌细胞直径和心肌细胞横截面积分别下降12.5%和10.8%（P<0.05）；（3）与心肌肥大组相比，治疗组心肌间质胶原容积分数和心肌羟脯氨酸含量分别下降22.3%和31.3%（P<0.05）。H₂S在降低左心室质量分数和心肌胶原水平、改善心肌肥大和减少心肌纤维化方面具有良好的临床疗效。值得进一步进行临床研究。
