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通过聚合酶链反应检测骨髓增生异常综合征和白血病中N-ras癌基因的突变

Mutations of N-ras oncogene in myelodysplastic syndromes and leukemias detected by polymerase chain reaction.

作者信息

Mano H, Ishikawa F, Hirai H, Takaku F

机构信息

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.

出版信息

Jpn J Cancer Res. 1989 Feb;80(2):102-6. doi: 10.1111/j.1349-7006.1989.tb02274.x.

DOI:10.1111/j.1349-7006.1989.tb02274.x
PMID:2498243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5917694/
Abstract

By using polymerase chain reaction and differential hybridization, point mutations of N-ras oncogene were investigated among 43 patients with hematopoietic malignancies. Six cases were revealed to carry mutational N-ras oncogenes. One case with acute myelocytic leukemia was found to carry a mutation at codon 13, although this case was in complete remission.

摘要

通过聚合酶链反应和差异杂交技术，对43例造血系统恶性肿瘤患者的N-ras癌基因点突变进行了研究。发现6例携带N-ras癌基因突变。1例急性髓细胞白血病患者虽处于完全缓解期，但发现其第13密码子存在突变。

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Mutations of N-ras oncogene in myelodysplastic syndromes and leukemias detected by polymerase chain reaction.通过聚合酶链反应检测骨髓增生异常综合征和白血病中N-ras癌基因的突变
Jpn J Cancer Res. 1989 Feb;80(2):102-6. doi: 10.1111/j.1349-7006.1989.tb02274.x.
2
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引用本文的文献

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2
Point mutation in codon 61 of N-RAS genes in human myeloma cell lines.人类骨髓瘤细胞系中N-RAS基因第61密码子的点突变。
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本文引用的文献

1
An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.一个激活的rasN基因：在传代后期而非早期的人PA1畸胎瘤细胞中检测到。
Science. 1984 Aug 10;225(4662):643-5. doi: 10.1126/science.6740333.
2
Structure and activation of the human N-ras gene.人类N-ras基因的结构与激活
Cell. 1983 Sep;34(2):581-6. doi: 10.1016/0092-8674(83)90390-2.
3
Isolation of genomic DNA.基因组DNA的分离
Methods Enzymol. 1987;152:180-3. doi: 10.1016/0076-6879(87)52018-3.
4
Activation mechanism of the N-ras oncogene in human leukemias detected by synthetic oligonucleotide probes.用合成寡核苷酸探针检测人类白血病中N-ras癌基因的激活机制。
Biochem Biophys Res Commun. 1987 Sep 15;147(2):870-5. doi: 10.1016/0006-291x(87)91010-2.
5
A point mutation at codon 13 of the N-ras oncogene in myelodysplastic syndrome.骨髓增生异常综合征中N-ras癌基因第13密码子处的点突变。
Nature. 1987;327(6121):430-2. doi: 10.1038/327430a0.
6
Mutation of Ki-ras and N-ras oncogenes in myelodysplastic syndromes.
Blood. 1988 Jun;71(6):1707-12.
7
Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes.通过聚合酶链反应和寡核苷酸探针分析急性髓系白血病中的RAS基因突变
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1629-33. doi: 10.1073/pnas.85.5.1629.
8
RAS mutations in myelodysplasia detected by amplification, oligonucleotide hybridization, and transformation.通过扩增、寡核苷酸杂交和转化检测到的骨髓增生异常综合征中的RAS突变
Leukemia. 1988 Aug;2(8):503-10.
9
RAS gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes.急性和慢性粒细胞白血病、慢性骨髓增殖性疾病及骨髓增生异常综合征中的RAS基因突变
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9228-32. doi: 10.1073/pnas.84.24.9228.
10
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.