Xu Hongxia, Ma Zhihong, Yang Ruibin, Xie Lingpeng, Yang Xuefen
a Key Lab of Freshwater Animal Breeding certificated by Ministry of Agriculture , College of Fisheries, Huazhong Agricultural University , Wuhan , China and.
b Freshwater Aquaculture Collaborative innovation Center of Hubei Province , Wuhan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1105-6. doi: 10.3109/19401736.2014.933321. Epub 2014 Jul 1.
In this study, we cloned and sequenced the complete mitochondrial genome of Macropodus ocellatus. The genome is a circular molecule of 16,501 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which were similar to most other vertebrates. All protein-coding genes are initiated with ATG except for COX 1, which begin with GTG instead. However, the termination codons of 13 protein-coding genes are varied with TAA, TA, T or AGG. This mitogenome sequenc data would contribute to better understanding species identification, evolutionary relationships and population genetics of the Macropodus.
在本研究中,我们克隆并测序了圆尾斗鱼的完整线粒体基因组。该基因组是一个长度为16,501 bp的环状分子,由13个蛋白质编码基因、22个tRNA基因、2个核糖体RNA基因和2个主要非编码区(控制区和轻链复制起点)组成。其基因组成和顺序与大多数其他脊椎动物相似。除COX 1以GTG起始外,所有蛋白质编码基因均以ATG起始。然而,13个蛋白质编码基因的终止密码子各不相同,分别为TAA、TA、T或AGG。该线粒体基因组序列数据将有助于更好地理解圆尾斗鱼的物种鉴定、进化关系和群体遗传学。