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粉红小丑鱼(双锯鱼)的线粒体全基因组(鱼类:鲈形目,雀鲷科)

Complete mitochondrial genome of the pink clownfish Amphiprion perideraion (Pisces: Perciformes, Pomacentridae).

作者信息

Hu Xueyi, Li Jianlong, Liu Min

机构信息

a College of Ocean and Earth Sciences, Xiamen University , Xiamen , Fujian , P. R. China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1135-6. doi: 10.3109/19401736.2014.933336. Epub 2014 Jul 1.

Abstract

In this study the complete mitochondrial (mt) genome of the pink clownfish Amphiprion perideraion was obtained by using eight consensus primer pairs with a long PCR technique. The circular mtDNA molecule was 16,579 bp in size and the overall nucleotide composition of the H-strand was 29.37% A, 25.50% T, 15.68% G and 29.45% C, with an A + T bias. The complete mitogenome contained 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a control region, and the gene order was typical of vertebrate mitogenomes. The complete mitochondrial genome of A. perideraion is a representative of the subgenus Phalerebus for mitogenomes database of anemonefishes, which can be used to unveil taxonomic problems and phylogenetic relationships in Amphiprioninae.

摘要

在本研究中,通过使用8对共有引物对和长PCR技术获得了粉红小丑鱼(Amphiprion perideraion)的完整线粒体(mt)基因组。环状线粒体DNA分子大小为16,579 bp,重链的总体核苷酸组成为29.37% A、25.50% T、15.68% G和29.45% C,具有A+T偏向性。完整的线粒体基因组包含13个蛋白质编码基因、2个rRNA、22个tRNA和一个控制区,基因顺序是脊椎动物线粒体基因组的典型顺序。A. perideraion的完整线粒体基因组是海葵鱼线粒体基因组数据库中Phalerebus亚属的一个代表,可用于揭示双锯鱼亚科的分类学问题和系统发育关系。

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