Andrade João Marcus Oliveira, Paraíso Alanna Fernandes, de Oliveira Marcos Vinícius Macedo, Martins Andréa Maria Eleutério, Neto João Felício, Guimarães André Luiz Sena, de Paula Alfredo Maurício, Qureshi Mahboob, Santos Sérgio Henrique Sousa
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Nursing Department, Faculdades Integradas Pitágoras, Montes Claros, Minas Gerais, Brazil.
Nutrition. 2014 Jul-Aug;30(7-8):915-9. doi: 10.1016/j.nut.2013.11.016. Epub 2013 Dec 4.
Resveratrol (RSV) is the most studied natural compound that activates sirtuins, which produce beneficial metabolic effects on lipid and glucose metabolism. The aim of the present study was to investigate the role of resveratrol in preventing nonalcoholic fatty liver disease (NAFLD) and expression of liver inflammatory markers in mice treated with a high-fat diet.
Eighteen male mice were divided into three groups and fed for 60 d with a standard diet (ST), high-fat diet (HFD), or high-fat diet plus resveratrol (HFD + RSV, 30 mg/kg/d). Body weight, food intake, and serum total cholesterol, triacylglycerol, insulin, alanine transaminase (ALT), and aspartate aminotransferase (AST) were evaluated. Liver histology was analyzed. Expression of ACC, PPAR-γ, ChREBP, SREBP-1 c, CPT-1, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), NF-κB, interleukin 1 β (IL-1 β), and SIRT1 were evaluated by quantitative real-time reverse transcriptase PCR (qRT-PCR).
The major finding of the present study was that RSV reduced body fat, total cholesterol, triacylglycerol, transaminases, and insulin plasma level. These results were accompanied with a significant reduction in TNF-α, IL-6, and NF-κB mRNA expression in the liver. Analyses of liver adipogenesis related genes indicated that ACC, PPAR-γ, and SREBP-1 mRNA expression were significantly suppressed in HFD + RSV mice. In addition, we observed increased expression of SIRT1 in the HFD + RSV group.
We observed that treatment with resveratrol improved lipid metabolism, and decreased NAFLD and pro-inflammatory profile in liver of mice with obesity-inducible diets. These data suggest an important clinical application of RSV in preventing liver diseases.
白藜芦醇（RSV）是研究最多的激活去乙酰化酶的天然化合物，其对脂质和葡萄糖代谢产生有益的代谢作用。本研究的目的是探讨白藜芦醇在预防非酒精性脂肪性肝病（NAFLD）中的作用以及在高脂饮食喂养的小鼠中肝脏炎症标志物的表达。
将18只雄性小鼠分为三组，分别用标准饮食（ST）、高脂饮食（HFD）或高脂饮食加白藜芦醇（HFD + RSV，30 mg/kg/d）喂养60天。评估体重、食物摄入量以及血清总胆固醇、三酰甘油、胰岛素、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）。分析肝脏组织学。通过定量实时逆转录聚合酶链反应（qRT-PCR）评估ACC、PPAR-γ、ChREBP、SREBP-1c、CPT-1、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、NF-κB、白细胞介素1β（IL-1β）和SIRT1的表达。
本研究的主要发现是RSV降低了体脂、总胆固醇、三酰甘油、转氨酶和胰岛素血浆水平。这些结果伴随着肝脏中TNF-α、IL-6和NF-κB mRNA表达的显著降低。肝脏脂肪生成相关基因的分析表明，HFD + RSV小鼠中ACC、PPAR-γ和SREBP-1 mRNA表达被显著抑制。此外，我们观察到HFD + RSV组中SIRT1的表达增加。
我们观察到白藜芦醇治疗改善了脂质代谢，并降低了肥胖诱导饮食小鼠肝脏中的NAFLD和促炎状态。这些数据表明RSV在预防肝脏疾病方面具有重要的临床应用价值。
