Choi Won Hee, Um Min Young, Ahn Jiyun, Jung Chang Hwa, Ha Tae Youl
Metabolism and Nutrition Research Group, Korea Food Research Institute, Seongnam, Republic of Korea; Division of Food Biotechnology, University of Science and Technology, Daejeon, Republic of Korea.
Metabolism and Nutrition Research Group, Korea Food Research Institute, Seongnam, Republic of Korea.
Nutrition. 2014 Jul-Aug;30(7-8):920-7. doi: 10.1016/j.nut.2013.12.021. Epub 2014 Jan 10.
The aim of this study was to evaluate the effects of rice as a carbohydrate source and its molecular mechanisms on insulin resistance induced by a high-fat diet (HFD).
C57 BL/6 J mice were divided into three groups and were fed a low-fat diet (LFD); a HFD (with 18% fat, 0.5% cholesterol, 51.5% w/w cornstarch and sucrose); or a HFD with rice (HFD-CR, with 18% fat, 0.5% cholesterol and 51.5% w/w rice powder) for 12 wk. In the HFD-CR diet, cooked rice powder was substituted for cornstarch and sucrose in the HFD as a carbohydrate source.
HFD-CR-fed mice had significantly lower body weight, blood glucose, insulin and leptin levels and ameliorated glucose responses with decreased homeostasis model assessment-insulin resistance compared with HFD-fed mice. Hepatic mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were down-regulated in the HFD-CR group. The hypertrophied islet size and the decreased pancreatic mRNA expression of glucose transporter 2 in the HFD group were normalized with cooked rice consumption. Rice promoted glucose uptake by activating AMP-activated protein kinase and downstream glucose transporter 4 in the skeletal muscle.
Rice consumption as a carbohydrate source might potentiate improvements in glucose uptake via AMP-activated protein kinase activation and glucose transporter 4 expression in the skeletal muscles, thereby improving insulin sensitivity.
本研究旨在评估大米作为碳水化合物来源对高脂饮食（HFD）诱导的胰岛素抵抗的影响及其分子机制。
将C57 BL/6 J小鼠分为三组，分别给予低脂饮食（LFD）；高脂饮食（含18%脂肪、0.5%胆固醇、51.5% w/w玉米淀粉和蔗糖）；或高脂大米饮食（HFD-CR，含18%脂肪、0.5%胆固醇和51.5% w/w米粉），持续12周。在HFD-CR饮食中，用熟米粉替代HFD中的玉米淀粉和蔗糖作为碳水化合物来源。
与高脂饮食喂养的小鼠相比，高脂大米饮食喂养的小鼠体重、血糖、胰岛素和瘦素水平显著降低，葡萄糖反应改善，稳态模型评估-胰岛素抵抗降低。高脂大米饮食组肝磷酸烯醇式丙酮酸羧激酶和葡萄糖-6-磷酸酶的mRNA水平下调。食用熟大米可使高脂饮食组胰岛肥大的大小和胰腺葡萄糖转运蛋白2 mRNA表达的降低恢复正常。大米通过激活骨骼肌中的AMP活化蛋白激酶和下游葡萄糖转运蛋白4促进葡萄糖摄取。
作为碳水化合物来源食用大米可能通过激活AMP活化蛋白激酶和骨骼肌中葡萄糖转运蛋白-4的表达来增强葡萄糖摄取的改善，从而提高胰岛素敏感性。
