College of Agronomy, Inner Mongolia Agricultural University , Hohhot 010019 , China.
Breed Sci. 2014 Jun;64(2):156-63. doi: 10.1270/jsbbs.64.156. Epub 2014 Jun 1.
A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani. Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After inoculation with pathogen discs and culture for 48 h, the necrotic spots on the inoculated potato pieces were generated and measured by the crossing method. Further evaluation was conducted through field experiments using a wheat bran inoculum method. The wheat bran inoculum was placed into the pit dispersedly and surrounded seed tubers. Each cultivar or line was subjected to five treatments of 0-, 2-, 3-, 4-, and 5-g soil inoculum. The results showed that 2-4 g of wheat bran inoculum was the optimum for identifying tuber black scurf resistance. The laboratory scores positively correlated with the incidence and severity of black scurf in the field. According to the results in the laboratory, relatively resistant cultivars could be selected for further estimation of tuber black scurf resistance in field experiments. It is a practical and effective screening method for rapid identification of resistant potato germplasm, which can reduce workload in the field, shorten time required for identification.
本研究建立了一种两步法来评估马铃薯对由立枯丝核菌引起的黑疤病的抗性。首先在实验室中进行薯块接种，这在本研究中也是首次报道。接种病原菌圆盘并培养 48 小时后，通过划线法产生并测量接种马铃薯块上的坏死斑。进一步通过使用麦麸接种的田间试验进行评估。将麦麸接种物分散放置在坑中，并环绕种薯。每个品种或系进行 0、2、3、4 和 5 g 土壤接种物的 5 种处理。结果表明，2-4 g 的麦麸接种物是鉴定块茎黑疤病抗性的最佳选择。实验室评分与田间黑疤病的发病率和严重度呈正相关。根据实验室的结果，可以选择相对抗性的品种进行田间试验进一步评估块茎黑疤病抗性。这是一种快速鉴定抗性马铃薯种质资源的实用有效筛选方法，可以减少田间工作量，缩短鉴定所需的时间。
