Ghafourian Boroujerdnia Mehri, Khosravi Neda, Malek-Hosseini Saeed, Amirghofran Zahra
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran .
Pharm Biol. 2014 Nov;52(11):1471-7. doi: 10.3109/13880209.2014.898077. Epub 2014 Jul 2.
Euphorbia is an important Euphorbiaceae genus that is traditionally being used for various infections, inflammation, and cancer.
The present study investigated the possible in vitro immunomodulatory effect of three species of Euphorbia genus including Euphorbia microciadia Boiss, Euphorbia osyridea Boiss, and Euphorbia heteradenia Jaub. & Sp. on lymphocyte activation and cytokine secretion.
Human lymphocytes were cultured in the presence of various concentrations (0.1-200 µg/ml) of the butanol/hexane extracts of the plants in the presence or absence of phytohemmagglutinin (PHA). The activation of lymphocytes after 48 h was determined by a proliferation assay. The release of T cell cytokines was studied to determine the dominant T cell subsets involved in the immune response.
All three plant extracts increased the proliferation of PHA-treated lymphocytes (maximum; 132% of control). Extract treatment of lymphocytes in the absence of PHA resulted in an increased proliferation of the cells indicating their lymphocyte mitogenic activity (maximum at 10 µg/ml E. microciadia extract; 494.5 ± 42.2% of control, p < 0.01). The extracts of E. microciadia and E. osyridea could increase IL-4 and IL-10 secretion but not IFN-γ production showing their capacity to deviate immune response toward a Th2 pattern. Euphorbia heteradenia did not change the release of IL-4 and IFN-γ cytokines but increased IL-10 production. The three extracts stimulated lymphocytes to produce IL-17 which showed their possible effects on Th17 cells activation.
The studied extracts had the ability to modulate T cell responses suggesting their possible beneficial effects on immune host defense.
大戟属是大戟科的一个重要属，传统上用于治疗各种感染、炎症和癌症。
本研究调查了三种大戟属植物，即微小大戟（Euphorbia microciadia Boiss）、奥西大戟（Euphorbia osyridea Boiss）和异腺大戟（Euphorbia heteradenia Jaub. & Sp.）对淋巴细胞活化和细胞因子分泌的体外免疫调节作用。
在有或无植物血凝素（PHA）存在的情况下，将人淋巴细胞与不同浓度（0.1 - 200μg/ml）的植物丁醇/己烷提取物一起培养。48小时后通过增殖试验测定淋巴细胞的活化情况。研究T细胞细胞因子的释放，以确定参与免疫反应的主要T细胞亚群。
所有三种植物提取物均增加了PHA处理的淋巴细胞的增殖（最大值为对照的132%）。在无PHA的情况下用提取物处理淋巴细胞导致细胞增殖增加，表明其淋巴细胞促有丝分裂活性（微小大戟提取物在10μg/ml时最高；为对照的494.5±42.2%，p < 0.01）。微小大戟和奥西大戟的提取物可增加IL - 4和IL - 10的分泌，但不增加IFN - γ的产生，表明它们有能力使免疫反应偏向Th2模式。异腺大戟不改变IL - 4和IFN - γ细胞因子的释放，但增加IL - 10的产生。这三种提取物刺激淋巴细胞产生IL - 17，表明它们可能对Th17细胞活化有影响。
所研究的提取物具有调节T细胞反应的能力，表明它们可能对免疫宿主防御有有益作用。
