Namdari Haideh, Izad Maryam, Amirghofran Zahra
Department of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Immunol. 2017 Jun;14(2):134-150.
Euphorbia plants are traditionally used in folk medicine for infections, inflammation, and cancer.
To investigate the effects of the butanolic extracts of Euphorbia micorociadia and Euphorbia osyridea on specific transcription factors and cytokines expression of T cell subsets.
Activated mouse splenocytes were cultured in the presence of non-cytotoxic concentrations of the extracts. Cells were evaluated for the gene expressions of T cell transcription factors and cytokines of T helper (Th)1 [T-bet and interferon gamma (IFNγ)], Th17 [retinoic acid receptor related orphan receptor (RORγt) and interleukin (IL)-17], and T regulatory (Treg) cells [forkhead box P3(Foxp3), IL-10, and Transforming growth factor (TGF)-β] using real-time PCR. The cytokine secretions were evaluated by ELISA and Foxp3 protein expression by flow cytometry.
Both E. osyridea and E. microciadia extracts at 0.1 μg/ml increased T-bet expression [>1.73 relative fold change (RFC), p<0.05] and IFNγ production (>1195 pg/ml, p<0.001). Both decreased Foxp3 (<0.41 RFC, p<0.05) expression. At the higher concentration both extracts significantly reduced T-bet mRNA as well as IFNγ, IL-17, IL-10, and TGF-β cytokines and Foxp3 at the mRNA and protein levels.
These data showed the immunomodulatory effects of E. osyridea and E. micorociadia extracts on T cell-mediated responses. The extracts caused upregulation of Th1 and downregulation of Treg cells at a low concentration which suggested their possible therapeutic value in tumor models and infectious diseases. The observed immunosuppressive effects at the higher concentration potentially make these plants candidates for identification of active components and studying their mechanisms of action.
大戟属植物在传统民间医学中用于治疗感染、炎症和癌症。
研究微小大戟和欧西瑞大戟的丁醇提取物对T细胞亚群特异性转录因子和细胞因子表达的影响。
在无细胞毒性浓度的提取物存在下培养活化的小鼠脾细胞。使用实时PCR评估细胞中T细胞转录因子以及辅助性T细胞（Th）1[T盒转录因子（T-bet）和干扰素γ（IFNγ）]、Th17[维甲酸受体相关孤儿受体（RORγt）和白细胞介素（IL）-17]和调节性T细胞（Treg）[叉头框蛋白P3（Foxp3）、IL-10和转化生长因子（TGF）-β]的细胞因子基因表达。通过酶联免疫吸附测定（ELISA）评估细胞因子分泌情况，通过流式细胞术评估Foxp3蛋白表达。
0.1μg/ml浓度的欧西瑞大戟和微小大戟提取物均增加了T-bet表达[相对倍数变化（RFC）>1.73，p<0.05]和IFNγ产生（>1195 pg/ml，p<0.001）。两者均降低了Foxp3（RFC<0.41，p<0.05）表达。在较高浓度下，两种提取物均显著降低了T-bet mRNA以及IFNγ、IL-17、IL-10和TGF-β细胞因子以及mRNA和蛋白水平的Foxp3。
这些数据显示了欧西瑞大戟和微小大戟提取物对T细胞介导反应的免疫调节作用。提取物在低浓度下导致Th1上调和Treg细胞下调，这表明它们在肿瘤模型和传染病中可能具有治疗价值。在较高浓度下观察到的免疫抑制作用可能使这些植物成为鉴定活性成分及其作用机制研究的候选对象。
