Zhu Lei, Zhao Xiao-Ying, Qiu Xi
Department of Clinical Laboratorial Examination, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.
Department of Hematology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Jun;22(3):617-22. doi: 10.7534/j.issn.1009-2137.2014.03.008.
This study was purposed to investigate the relationship between sequential changes of bone mineral density and bone marrow hematopoiesis in ovariectomized rats, and explore the correlation between the osteoporosis and the hematopoiesis. Fifty female Sprege-Dawley rats aged 3 months were randomly divided into ovariectomized (OVX) and sham operated (Sham) groups, euthanized after 4, 8, 12, 16 and 20 weeks respectively. The bone mineral density (BMD) of left femur was measured. The right femur distal metaphysical cancellous bone was fixed in 10% formalin to observe the changes of the bone marrow pathology. The function of hematopoietic stem cells (HSC) was evaluated by colony forming assay. The level of granulocyte macrophage-colony stimulating factor (GM-CSF) was measured by the enzyme linked immunosorbent assay (ELISA). The results showed that the BMD of femur in OVX 4 week group not decreased significantly as compared with the Sham 4 group, but the volume of adipose tissue significantly increased. The BMD of femur in OVX 8, 12, 16 and 20 weeks decreased significantly as compared with the corresponding Sham groups (P < 0.05). Meanwhile, the volume of hematopoietic tissue decreased and volume of adipose tissue increased, the megakaryocyte number decreased, the number of osteoclasts and mast cells increased in bone marrow section, as compared with sham operated group (P < 0.05). After rats were ovariectomized for 16 weeks, the GM-CSF level was significantly lower than that of Sham group. After rats were ovariectomized for 12 weeks, the GM-CSF level was obviously lower than that of Sham group (P < 0.05). It is concluded that the bone marrow hematopoiesis function also decreases when the BMD of ovariectomized rats reduces. The Sprege-Dawley OVX rats aged 3 months can be used as an model to study the hypohemopoiesis.
本研究旨在探讨去卵巢大鼠骨密度的序贯变化与骨髓造血之间的关系，并探究骨质疏松与造血之间的相关性。将50只3月龄雌性斯普拉格-道利大鼠随机分为去卵巢（OVX）组和假手术（Sham）组，分别在4、8、12、16和20周后实施安乐死。测量左股骨的骨密度。将右股骨远端干骺端松质骨固定于10%福尔马林中，以观察骨髓病理学变化。通过集落形成试验评估造血干细胞（HSC）的功能。采用酶联免疫吸附测定（ELISA）法检测粒细胞巨噬细胞集落刺激因子（GM-CSF）水平。结果显示，OVX 4周组大鼠股骨骨密度与Sham 4组相比无显著降低，但脂肪组织体积显著增加。OVX 8、12、16和20周组大鼠股骨骨密度与相应的Sham组相比显著降低（P<0.05）。同时，与假手术组相比，骨髓切片中造血组织体积减少，脂肪组织体积增加，巨核细胞数量减少，破骨细胞和肥大细胞数量增加（P<0.05）。大鼠去卵巢16周后，GM-CSF水平显著低于Sham组。大鼠去卵巢12周后，GM-CSF水平明显低于Sham组（P<0.05）。结论是，去卵巢大鼠骨密度降低时，骨髓造血功能也随之下降。3月龄的斯普拉格-道利去卵巢大鼠可作为研究造血功能减退的模型。
