Chang Wen-Lin, Yang Qing, Zhang Hui, Lin Hai-Yan, Zhou Zhi, Lu Xiaoyin, Zhu Cheng, Xue Li-Qun, Wang Hongmei
College of Veterinary MedicineHunan Agricultural University, Changsha 410128, ChinaState Key Laboratory of Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaBeijing Obstetrics and Gynecology HospitalCapital Medical University, Beijing 100069, ChinaGraduate School of Chinese Academy of SciencesBeijing 100039, China College of Veterinary MedicineHunan Agricultural University, Changsha 410128, ChinaState Key Laboratory of Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaBeijing Obstetrics and Gynecology HospitalCapital Medical University, Beijing 100069, ChinaGraduate School of Chinese Academy of SciencesBeijing 100039, China.
College of Veterinary MedicineHunan Agricultural University, Changsha 410128, ChinaState Key Laboratory of Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaBeijing Obstetrics and Gynecology HospitalCapital Medical University, Beijing 100069, ChinaGraduate School of Chinese Academy of SciencesBeijing 100039, China.
Reproduction. 2014 Oct;148(4):343-52. doi: 10.1530/REP-14-0052. Epub 2014 Jul 2.
Placenta-specific protein 1 (PLAC1), a placenta-specific gene, is known to be involved in the development of placenta in both humans and mice. However, the precise role of PLAC1 in placental trophoblast function remains unclear. In this study, the localization of PLAC1 in human placental tissues and its physiological significance in trophoblast invasion and migration are investigated by technical studies including real-time RT-PCR, in situ hybridization, immunohistochemistry, and functional studies by utilizing cell invasion and migration assays in the trophoblast cell line HTR8/SVneo as well as the primary inducing extravillous trophoblasts (EVTs). The results show that PLAC1 is mainly detected in the trophoblast columns and syncytiotrophoblast of the first-trimester human placental villi, as well as in the EVTs that invade into the maternal decidua. Knockdown of PLAC1 by RNA interference significantly suppresses the invasion and migration of HTR8/SVneo cells and shortens the distance of the outgrowth of the induced EVTs from the cytotrophoblast column of the explants. All the above data suggests that PLAC1 plays an important role in human placental trophoblast invasion and migration.
胎盘特异性蛋白1（PLAC1）是一种胎盘特异性基因，已知其在人类和小鼠的胎盘发育过程中发挥作用。然而，PLAC1在胎盘滋养层细胞功能中的具体作用仍不清楚。在本研究中，通过实时逆转录聚合酶链反应（RT-PCR）、原位杂交、免疫组织化学等技术研究，以及利用滋养层细胞系HTR8/SVneo和原代诱导绒毛外滋养层细胞（EVT）进行细胞侵袭和迁移试验的功能研究，探讨了PLAC1在人胎盘组织中的定位及其在滋养层细胞侵袭和迁移中的生理意义。结果显示，PLAC1主要在孕早期人胎盘绒毛的滋养层柱和合体滋养层中检测到，也在侵入母体蜕膜的EVT中检测到。通过RNA干扰敲低PLAC1可显著抑制HTR8/SVneo细胞的侵袭和迁移，并缩短外植体中诱导的EVT从细胞滋养层柱长出的距离。上述所有数据表明，PLAC1在人胎盘滋养层细胞的侵袭和迁移中起重要作用。
