Chang Wen-Lin, Wang Huiying, Cui Lina, Peng Nan-Ni, Fan Xiujun, Xue Li-Qun, Yang Qing
College of Veterinary Medicine, Hunan Agricultural University, Changsha, China; State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKUHKUST Medical Center, Shenzhen, China.
Beijing Shijitan Hospital, Capital Medical University, Beijing, China; Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
Reprod Biol. 2016 Sep;16(3):218-224. doi: 10.1016/j.repbio.2016.07.001. Epub 2016 Jul 16.
Placenta specific protein 1 (PLAC1) is thought to be important for murine and human placentation because of its abundant expression in placenta; however, the trophoblast subtypes that express PLAC1 at the fetomaternal interface and the major role of PLAC1 in placentation are still unclear. This study investigated the expression pattern of PLAC1 at the human fetomaternal interface and its involvement in trophoblast syncytialization. Localization of PLAC1 at the fetomaternal interface was studied using in situ hybridization (ISH) and immunohistochemistry (IHC) assays. Real time RT-PCR and Western Blot were employed to exhibit the expression pattern of PLAC1 during human spontaneous syncytialization of term primary cytotrophoblast cells (CTBs). Spontaneous syncytialization of a primary term CTBs model transfected with siRNA specific to PLAC1 was used to investigate the role of PLAC1 during human trophoblast syncytialization. The results showed that PLAC1 was mainly expressed in the human villous syncytiotrophoblast (STB) layer throughout gestation, and the expression level of PLAC1 was significantly elevated during human trophoblast syncytialization. Down-regulation of PLAC1 via specific PLAC1 siRNA transfection attenuated spontaneous syncytialization of primary term CTBs (p<0.05) as indicated by cell fusion index and the expression patterns of the corresponding markers. These data demonstrate the facilitative role of PLAC1 in normal human trophoblast syncytialization.
胎盘特异性蛋白1（PLAC1）因其在胎盘中的丰富表达而被认为对小鼠和人类的胎盘形成很重要；然而，在母胎界面表达PLAC1的滋养层细胞亚型以及PLAC1在胎盘形成中的主要作用仍不清楚。本研究调查了PLAC1在人类母胎界面的表达模式及其在滋养层细胞融合中的作用。使用原位杂交（ISH）和免疫组织化学（IHC）分析研究了PLAC1在母胎界面的定位。采用实时RT-PCR和蛋白质免疫印迹法展示足月原代细胞滋养层细胞（CTB）在人类自然融合过程中PLAC1的表达模式。利用针对PLAC1的特异性siRNA转染的足月原代CTB模型的自然融合来研究PLAC1在人类滋养层细胞融合过程中的作用。结果表明，PLAC1在整个妊娠期主要表达于人类绒毛合体滋养层（STB）层，并且在人类滋养层细胞融合过程中PLAC1的表达水平显著升高。如细胞融合指数和相应标志物的表达模式所示，通过特异性PLAC1 siRNA转染下调PLAC1可减弱足月原代CTB的自然融合（p<0.05）。这些数据证明了PLAC1在正常人类滋养层细胞融合中的促进作用。
