Tang Chunlei, Xie Desheng, Feng Bainian
School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
Chem Biol Drug Des. 2015 Mar;85(3):253-8. doi: 10.1111/cbdd.12389. Epub 2014 Jul 22.
The zebrafish-based assay is a widely used animal model system for cardiovascular research. In this study, we investigated the cardiac defects caused by terfenadine and tested the pharmacological response of digoxin to zebrafish with cardiac defects. The study suggested that zebrafish could be a suitable model for phenotype-based screening and evaluation of positive inotropic agents. This phenotype-based heart failure zebrafish model system was then utilized in in-house library screen. Some positive inotropic compound was discovered, which could attenuate the cardiac defects by increasing the flow velocity of caudal artery blood.
基于斑马鱼的检测方法是心血管研究中广泛使用的动物模型系统。在本研究中，我们研究了特非那定引起的心脏缺陷，并测试了地高辛对有心脏缺陷斑马鱼的药理反应。该研究表明，斑马鱼可能是基于表型筛选和评估正性肌力药物的合适模型。然后，这种基于表型的心力衰竭斑马鱼模型系统被用于内部文库筛选。发现了一些正性肌力化合物，它们可以通过增加尾动脉血流速度来减轻心脏缺陷。
