Barbouch M R, Gorgi Y, Ayed K, Haddad S, Ben Ayed H
Service de Médecine, Hôpital Charles-Nicolle, Tunis.
Rev Rhum Mal Osteoartic. 1989 Apr;56(5):389-94.
The clinical significance of antibodies directed against extractable nuclear antigens with currently well-known antigenic targets, is studied in 100 patients affected with various connective tissue inflammations. The anti-Sm is the most frequently encountered antibody in lupus disease and overlapping connective tissue inflammations with a lupus component while the anti-RNP is related to mixed inflammation of the connective tissue and the dry syndrome. These antibodies, without being specific markers for a given disease, represent an additional biological argument for a better nosological classification of connective tissue inflammations. The use of immuno-impression, a more sensitive technique than immunodiffusion, could provide a better understanding of the immune response during these diseases and specify the diagnostic and prognostic value of various auto-antibodies. Analysis by immuno-impression of 36 sera among the 100 with antibodies directed against extractable nuclear antigens has enabled us to compare the two techniques and study the different specificities of these antibodies.
在100例患有各种结缔组织炎症的患者中，研究了针对具有目前已知抗原靶点的可提取核抗原的抗体的临床意义。抗Sm抗体是狼疮疾病以及伴有狼疮成分的重叠性结缔组织炎症中最常遇到的抗体，而抗RNP抗体则与结缔组织混合炎症和干燥综合征相关。这些抗体虽然不是特定疾病的特异性标志物，但为结缔组织炎症更好的疾病分类提供了额外的生物学依据。免疫印迹法是一种比免疫扩散更敏感的技术，它可以更好地理解这些疾病中的免疫反应，并明确各种自身抗体的诊断和预后价值。通过对100例中有针对可提取核抗原抗体的36份血清进行免疫印迹分析，我们得以比较这两种技术，并研究这些抗体的不同特异性。
