[可提取核抗原多肽抗体：检测及其临床意义]

[Antibodies to extractable nuclear antigen polypeptides: detection and its clinical significance].

作者信息

Xu Y H

机构信息

Ren Ji Hospital, Shanghai Second Medical University.

出版信息

Zhonghua Yi Xue Za Zhi. 1992 Sep;72(9):541-3, 573.

PMID:1338517

Abstract

Using immunoblotting technique (IBT) and saline extracts of rabbit thymus powder as antigen, we detected seven autoantibodies i.e. anti-Sm, anti-RNP, anti-SSA, anti-SSB, anti-Scl-70, anti-Jo-1 and anti-ribosome with only a strip of blot. Comparison of IBT and counter-immunoelectrophoresis (CIE) showed that the antibodies against Sm in SLE could be detected more sensitively by IBT than by CIE (P < 0.01). And the antibodies against ribosome by IBT were much specific for SLE (P < 0.01). So the detection of antibodies against ENA polypeptides by IBT was helpful in the diagnosis of SLE. In rheumatic diseases, the antibodies against SSB could be detected more sensitively by IBT than by CIE (P < 0.05), while the antibodies against SSA could be detected more sensitively by CIE than by IBT (P < 0.01). The antibodies against Scl-70 and Jo-1 by IBT were much specific for PSS and PM/DM respectively (P < 0.01).

摘要

我们以兔胸腺粉的盐水提取物为抗原，采用免疫印迹技术（IBT）检测了七种自身抗体，即抗Sm、抗RNP、抗SSA、抗SSB、抗Scl - 70、抗Jo - 1和抗核糖体，印迹条带单一。免疫印迹技术（IBT）与对流免疫电泳（CIE）的比较表明，在系统性红斑狼疮（SLE）中，IBT检测抗Sm抗体比CIE更灵敏（P < 0.01）。并且IBT检测抗核糖体抗体对SLE具有高度特异性（P < 0.01）。因此，用IBT检测抗可提取核抗原（ENA）多肽抗体有助于SLE的诊断。在风湿性疾病中，IBT检测抗SSB抗体比CIE更灵敏（P < 0.05），而CIE检测抗SSA抗体比IBT更灵敏（P < 0.01）。IBT检测抗Scl - 70和抗Jo - 1抗体分别对进行性系统性硬化症（PSS）和多发性肌炎/皮肌炎（PM/DM）具有高度特异性（P < 0.01）。