Kazierad D J, Hoon T J, Bottorff M B
Department of Clinical Pharmacy, University of Tennessee, Memphis 38163.
Ther Drug Monit. 1989;11(3):327-31. doi: 10.1097/00007691-198905000-00016.
A high-performance liquid chromatography (HPLC) procedure used to quantitate encainide and two of its active metabolites, O-desmethylencainide (ODE) and 3-methoxy-O-desmethylencainide (MODE), is described. All three compounds were simultaneously extracted from urine and serum using an octyl (C-8) solid-phase extraction column. The compounds were then separated by reverse-phase HPLC on a cyanopropylsilane column using ultraviolet detection at 260 nm. Serum samples were quantified over a concentration range of 25-400 ng/ml and urine over a range of 150-10,000 ng/ml. Total run time for the assay was less than 12 min. Within-day and between-day precision and relative error were less than 10% in serum and less than 13% in urine for all three compounds. The lower limit of quantitation was 10 ng/ml for encainide and ODE and 15 ng/ml for MODE. This HPLC procedure represents a quick and reliable method of measuring encainide and its major metabolites in both urine and serum, making the assay applicable as an aid for therapeutic drug monitoring of patients receiving encainide therapy.
本文描述了一种用于定量测定恩卡尼及其两种活性代谢产物O-去甲基恩卡尼（ODE）和3-甲氧基-O-去甲基恩卡尼（MODE）的高效液相色谱（HPLC）方法。使用辛基（C-8）固相萃取柱从尿液和血清中同时提取这三种化合物。然后，在氰丙基硅烷柱上通过反相HPLC分离这些化合物，并在260nm处进行紫外检测。血清样品的定量浓度范围为25-400ng/ml，尿液的定量浓度范围为150-10,000ng/ml。该测定的总运行时间少于12分钟。对于所有三种化合物，日内和日间精密度以及相对误差在血清中小于10%，在尿液中小于13%。恩卡尼和ODE的定量下限为10ng/ml，MODE的定量下限为15ng/ml。这种HPLC方法是一种快速可靠的测定尿液和血清中恩卡尼及其主要代谢产物的方法，可作为接受恩卡尼治疗患者的治疗药物监测辅助手段。
