Turgeon J, Funck-Brentano C, Gray H T, Roden D M
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
J Chromatogr. 1989 May 5;490(1):165-74. doi: 10.1016/s0378-4347(00)82771-1.
Methods reported previously for the determination of encainide and its metabolites in biological fluids have not been extensively described and evaluated. We report an improved high-performance liquid chromatographic assay for the quantification of these compounds in plasma and urine with complete estimation of the accuracy and reproducibility of the analytical method. The major improvements consist of: (1) the use of ethaverine as an appropriate internal standard; (2) the use of the salting-out technique which improves the extraction recovery for the metabolites of encainide and the sensitivity of the assay; and (3) a shift of the ultraviolet absorption wavelength from 254 to 270 nm to increase the selectivity of the detection.
先前报道的用于测定生物流体中恩卡尼及其代谢物的方法尚未得到广泛描述和评估。我们报告了一种改进的高效液相色谱法，用于定量测定血浆和尿液中的这些化合物，并全面评估了分析方法的准确性和重现性。主要改进包括：（1）使用依他维林作为合适的内标；（2）采用盐析技术，提高了恩卡尼代谢物的提取回收率和测定灵敏度；（3）将紫外吸收波长从254nm 移至270nm，以提高检测的选择性。
