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超声对染料木悬浮培养物中异黄酮生成的影响。

Effect of ultrasound on the isoflavonoid production in Genista tinctoria L. suspension cultures.

作者信息

Tůmová Lenka, Tůma Jiří, Hendrychová Helena

机构信息

Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.

Department of Biology, University of Hradec Králové, Faculty of Natural Sciences, Rokitanského 62 500 03 Hradec Králové, Czech Republic.

出版信息

Pharmacogn Mag. 2014 Apr;10(Suppl 2):S425-9. doi: 10.4103/0973-1296.133297.

DOI:10.4103/0973-1296.133297
PMID:24991122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4078351/
Abstract

BACKGROUND

Application of ultrasound (US) to biotechnology is relatively new but several processes that take place in the presence of cells or enzymes are activated by ultrasonic waves. Genista tinctoria L. (Fabaceae) is rich on various kind of flavonoids, including isoflavones with valuable estrogenic activity.

OBJECTIVE

This study verified use of low-energy US elicitor to enhance secondary metabolite production in plant cell cultures.

MATERIALS AND METHODS

Suspension cultures of G. tinctoria cells was exposed to low-power US (with fixed frequency 35 kHz and power level 0.1 mW/cm(3)) for period 1-5 min.

RESULTS

The US exposure significantly stimulated genistin content (0.8 mg/g DW) after 3 min of US treatment (sampled after 72 h). The highest daidzein level (1.4 mg/g DW) was reached after US irradiation for 5 min and 168 h sampling.

CONCLUSION

The achieved results suggest that US can act as a potent abiotic elicitor to induce the defense responses of plant cells and to stimulate secondary metabolite production in plant cell cultures.

摘要

背景

超声（US）在生物技术中的应用相对较新，但在细胞或酶存在的情况下发生的一些过程会被超声波激活。染料木（豆科）富含各种黄酮类化合物，包括具有宝贵雌激素活性的异黄酮。

目的

本研究验证了使用低能量超声激发子来提高植物细胞培养物中次生代谢产物的产量。

材料与方法

将染料木细胞悬浮培养物暴露于低功率超声（固定频率35kHz，功率水平0.1mW/cm³）下1至5分钟。

结果

超声处理3分钟（72小时后取样）后，超声暴露显著刺激了染料木苷含量（0.8mg/g干重）。超声照射5分钟并在168小时取样后，达到了最高的大豆苷元水平（1.4mg/g干重）。

结论

所取得的结果表明，超声可以作为一种有效的非生物激发子，诱导植物细胞的防御反应并刺激植物细胞培养物中次生代谢产物的产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/0954e1fae671/PM-10-425-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/63d7d2fea765/PM-10-425-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/02199128d446/PM-10-425-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/d83946d93145/PM-10-425-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/0954e1fae671/PM-10-425-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/63d7d2fea765/PM-10-425-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/02199128d446/PM-10-425-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/d83946d93145/PM-10-425-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff66/4078351/0954e1fae671/PM-10-425-g004.jpg

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