Faggioni Giovanni, De Santis Riccardo, Pomponi Alice, Fantini Massimo, Savini Giovanni, Monaco Federica, Polci Andrea, Bei Roberto, Lista Florigio
Laboratory of Molecular Biology, Army Medical Research Center, Via Santo Stefano Rotondo 4, 00184 Rome, Italy.
Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
J Virol Methods. 2014 Oct;207:54-9. doi: 10.1016/j.jviromet.2014.06.020. Epub 2014 Jun 30.
Following its spread in the USA, West Nile Virus (WNV) has reemerged in the Mediterranean basin with a renewed pathogenicity. The introduction of WNV lineage 2 in Europe and its co-circulation with lineage 1 has resulted in a continuously changing epidemiological scenario, highlighting the importance of differential detection of the two lineages. The paper describes a new real-time PCR method for the detection and genotyping of the two main lineages of WNV. The method requires a single pair of primers and probes and is based on the analysis of highly conserved consensus sequences detected in the 5' terminus of the viral genome.
西尼罗河病毒（WNV）在美国传播之后，又在地中海盆地重新出现，且致病性有所增强。WNV 2型在欧洲的传入及其与1型的共同传播导致了流行病学情况不断变化，凸显了对这两个病毒株进行鉴别检测的重要性。本文描述了一种用于检测WNV两个主要病毒株并进行基因分型的新型实时PCR方法。该方法只需一对引物和探针，基于对在病毒基因组5'端检测到的高度保守共有序列的分析。
