Yang S Y, Kim J W, Lee S Y, Kang J H, Ulziisaikhan U, Yoo H I, Moon Y H, Moon J S, Ko H M, Kim M S, Kim S H
Dental Science Research Institute, Department of Oral Anatomy, School of Dentistry, Chonnam National University, Gwangju, 500-757, South Korea.
Clin Oral Investig. 2015 Apr;19(3):657-65. doi: 10.1007/s00784-014-1276-4. Epub 2014 Jul 5.
We have previously reported that relaxin (Rln) expression from the ovary is upregulated by orthodontic tooth movement. This study was performed to test the hypothesis that Rln family peptides (Rxfps), the G-protein-coupled Rln receptor, is induced in periodontal ligament (PDL) cells to modulate the molecules involved in periodontal tissue remodeling while applying biophysical force.
Rats were implanted with orthodontic appliances to investigate changes to Rxfps in vivo. An in vitro biophysical force analysis was performed to measure the level of Rxfp 1 messenger RNA (mRNA) in primary human PDL cells.
The levels of Rxfp 2 transcription and translation increased in a time-dependent manner during tooth movement. Rxfp 2 was localized in the PDL by immunofluorescence. In vitro analyses revealed that the level of Rxfp 1 mRNA in PDL cells increased significantly with both compression and tension force. The levels of matrix metalloproteinase (MMP)-1, MMP-2, interleukin-6, and vascular endothelial growth factor mRNA, which are important for periodontal tissue remodeling, also changed under force application and Rln treatment.
PDL cells responded to Rln to modulate effector molecules for periodontal tissue remodeling by upregulating Rxfps expression under a biophysical force.
Rln and Rxfps may serve as a PDL turnover molecule complex to control orthodontic tooth movement.
我们之前报道过，正畸牙齿移动可上调卵巢中松弛素（Rln）的表达。本研究旨在验证以下假设：在施加生物物理力时，牙周膜（PDL）细胞中会诱导G蛋白偶联的Rln受体——Rln家族肽（Rxfps），以调节参与牙周组织重塑的分子。
给大鼠植入正畸矫治器，以研究体内Rxfps的变化。进行体外生物物理力分析，以测量原代人PDL细胞中Rxfp 1信使核糖核酸（mRNA）的水平。
在牙齿移动过程中，Rxfp 2的转录和翻译水平呈时间依赖性增加。通过免疫荧光法发现Rxfp 2定位于牙周膜。体外分析显示，压缩力和拉力均可使PDL细胞中Rxfp 1 mRNA的水平显著增加。在施加力和Rln处理的情况下，对牙周组织重塑很重要的基质金属蛋白酶（MMP）-1、MMP-2、白细胞介素-6和血管内皮生长因子mRNA的水平也发生了变化。
在生物物理力作用下，PDL细胞通过上调Rxfps表达对Rln作出反应，以调节牙周组织重塑的效应分子。
Rln和Rxfps可能作为一种牙周膜更新分子复合物来控制正畸牙齿移动。
