Yang So-Young, Ko Hyun-Mi, Kang Jee-Hae, Moon Yeon-Hee, Yoo Hong-Il, Jung Na-Ri, Kim Min-Seok, Cho Jin-Hyung, Oh Won-Mann, Kim Sun-Hun
2nd Stage Brain Korea, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, South Korea.
Eur J Oral Sci. 2011 Apr;119(2):115-20. doi: 10.1111/j.1600-0722.2011.00814.x.
Relaxin (Rln) is an ovarian hormone that stimulates osteoclastic and osteoblastic activities and connective tissue turnover. To investigate the expression of Rln during orthodontic tooth movement, rats were implanted with orthodontic appliances that connected a spring from the upper incisors to the first molar with a 70 cN force. Rats in each group were killed 6, 48, and 144 h after activating the appliance, and the levels of Rln1 and Rln3 expression in the ovary were determined by real-time RT-PCR, northern blots, western blots, and immunofluorescence analyses. The amount of tooth movement induced by the orthodontic force increased in a time-dependent manner. The levels of Rln1 mRNA increased by 12-, 41-, and 263-fold at 6, 48, and 144 h, respectively, after orthodontic tooth movement. The time-dependent increase in the concentration of Rln 1 protein in the ovary was also confirmed by western blotting. Rln 1 was localized in the granulosa cells of the ovarian follicles, and the immunoreactivity against Rln 1 was increased by the movement. In contrast, the concentration of Rln 3 was below the level of detection. The results of this study suggest that local changes in periodontal tissues induced by orthodontic tooth movement may affect Rln1 expression in the ovary. However, further studies are needed to decipher the mechanisms involved and the possible contribution of the increased level of expression of Rln 1 to the tooth movement.
松弛素（Rln）是一种卵巢激素，可刺激破骨细胞和成骨细胞的活性以及结缔组织更新。为了研究正畸牙齿移动过程中Rln的表达情况，给大鼠植入正畸矫治器，该矫治器用70 cN的力将一根弹簧从上门牙连接到第一磨牙。在激活矫治器后的6、48和144小时处死每组大鼠，通过实时RT-PCR、Northern印迹、Western印迹和免疫荧光分析来测定卵巢中Rln1和Rln3的表达水平。正畸力引起的牙齿移动量呈时间依赖性增加。正畸牙齿移动后，Rln1 mRNA水平在6、48和144小时分别增加了12倍、41倍和263倍。Western印迹也证实了卵巢中Rln 1蛋白浓度随时间的增加。Rln 1定位于卵巢卵泡的颗粒细胞中，并且移动使其对Rln 1的免疫反应性增加。相比之下，Rln 3的浓度低于检测水平。本研究结果表明，正畸牙齿移动引起的牙周组织局部变化可能会影响卵巢中Rln1的表达。然而，需要进一步研究来阐明其中涉及的机制以及Rln 1表达水平升高对牙齿移动的可能作用。
