Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon 302-318, Republic of Korea.
University of Science and Technology, Daejeon 305-850, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3282-3287. doi: 10.1099/ijs.0.060749-0. Epub 2014 Jul 3.
A Gram-staining-negative, strictly aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated E-II-3(T), was isolated from ground water at Daejeon in Korea. Strain E-II-3(T) grew between 4 and 45 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0-2.0 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain E-II-3(T) was shown to belong to the genus Novosphingobium and showed closest phylogenetic similarity to 'Novosphingobium ginsenosidimutans' FW-6 (97.7 %), Novosphingobium aromaticivorans F199(T) (96.9 %) and Novosphingobium subterraneum B0478(T) (96.5 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were C18 : 1ω7c (34.0 %), C16 : 1ω7c and/or iso-C15 : 0 2-OH (23.8 %) and C17 : 1ω6c (19.3 %). The DNA G+C content of this novel isolate was 62.7 mol%. DNA-DNA relatedness between strain E-II-3(T) and 'N. ginsenosidimutans' KACC 16615, N. aromaticivorans KCTC 2888(T) and N. capsulatum KCTC 22844(T) was 38, 33 and 29 %, respectively. On the basis of polyphasic analysis from this study, strain E-II-3(T) represents a novel species of the genus Novosphingobium for which the name Novosphingobium aquiterrae sp. nov. is proposed. The type strain is E-II-3(T) ( = KACC 17599(T) = NBRC 109812(T) = NCAIM B 02537(T)).
一株革兰氏染色阴性、严格需氧、不运动、不产芽孢、黄色、杆状的细菌，被命名为 E-II-3(T)，从韩国大田的地下水分离得到。E-II-3(T)菌株在 4 到 45°C（最适生长温度为 28°C）、pH 值 6.0 到 9.0（最适生长 pH 值为 7.5）和 0-2.0%（w/v）NaCl 盐度下生长，在 0.5%（w/v）NaCl 中生长最佳。根据 16S rRNA 基因序列分析，E-II-3(T)菌株属于新鞘氨醇单胞菌属，与“新鞘氨醇单胞菌 ginsenosidimutans”FW-6（97.7%）、芳香环新鞘氨醇单胞菌 F199(T)（96.9%）和地底下新鞘氨醇单胞菌 B0478(T)（96.5%）亲缘关系最近。主要的极性脂是磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和神经酰胺糖脂。主要的醌和多胺成分分别是 Q-10 和亚精胺。主要脂肪酸是 C18:1ω7c（34.0%）、C16:1ω7c 和/或 iso-C15:0 2-OH（23.8%）和 C17:1ω6c（19.3%）。该新型分离株的 DNA G+C 含量为 62.7 mol%。E-II-3(T)菌株与“新鞘氨醇单胞菌 ginsenosidimutans”KACC 16615、芳香环新鞘氨醇单胞菌 KCTC 2888(T)和地底下新鞘氨醇单胞菌 KCTC 22844(T)的 DNA-DNA 同源性分别为 38%、33%和 29%。根据本研究的多相分析，E-II-3(T)菌株代表新鞘氨醇单胞菌属的一个新种，现命名为Aquiterrae 新鞘氨醇单胞菌 nov.sp.。模式菌株为 E-II-3(T)（=KACC 17599(T)=NBRC 109812(T)=NCAIM B 02537(T)）。
