Sheu Shih-Yi, Liu Li-Ping, Young Chiu-Chung, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2423-2429. doi: 10.1099/ijsem.0.001973. Epub 2017 Jul 21.
A bacterial strain designated STM-14T was isolated from a spring in Taiwan and characterized using a polyphasic taxonomic approach. Strain STM-14T was a Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, rod-shaped bacterium and formed cream-coloured colonies. Strain STM-14T grew at 15-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum, pH 6.0) and with 0-0.5 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain STM-14T belonged to the genus Novosphingobium and showed the highest levels of sequence similarity to Novosphingobium naphthae D39T (98.5 %) and Novosphingobium mathurense SM117T (97.6 %). The major fatty acids (>10 %) of strain STM-14T were C17 : 1ω6c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C18 : 1ω7c, C15 : 0 2-OH and C17 : 1ω8c. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 66.6 mol%. The DNA-DNA hybridization value for strain STM-14T with N. naphthae JCM 31158T and N. mathurense DSM 23374T was less than 49 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain STM-14T should be classified as representing a novel species of the genus Novosphingobium, for which the name Novosphingobium fontis sp. nov. is proposed. The type strain is STM-14T (=BCRC 80924T=LMG 29290T=KCTC 42983T).
从台湾一处温泉中分离出一株命名为STM-14T的细菌菌株，并采用多相分类方法对其进行了特征描述。菌株STM-14T为革兰氏阴性、需氧、聚-β-羟基丁酸积累型、无运动性的杆状细菌，形成奶油色菌落。菌株STM-14T在15-37 °C（最适温度为30 °C）、pH 5.0-9.0（最适pH为6.0）以及含0-0.5 %（w/v）NaCl（最适含量为0 %）的条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株STM-14T属于新鞘氨醇菌属，与萘新鞘氨醇菌D39T（98.5 %）和马图拉新鞘氨醇菌SM117T（97.6 %）的序列相似性最高。菌株STM-14T的主要脂肪酸（>10 %）为C17 : 1ω6c、特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）、C18 : 1ω7c、C15 : 0 2-OH和C17 : 1ω8c。极性脂质谱由磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰二甲基乙醇胺、鞘糖脂、磷脂酰胆碱和几种未鉴定的脂质组成。主要的多胺是亚精胺。主要的类异戊二烯醌是Q-10。DNA G+C含量为66.6 mol%。菌株STM-14T与萘新鞘氨醇菌JCM 31158T和马图拉新鞘氨醇菌DSM 23374T的DNA-DNA杂交值小于49 %。不同的表型特征以及系统发育推断表明，菌株STM-14T应被分类为新鞘氨醇菌属的一个新物种，为此提出新物种名为新鞘氨醇菌泉生种（Novosphingobium fontis sp. nov.）。模式菌株为STM-14T（=BCRC 80924T=LMG 29290T=KCTC 42983T）。
