Robinson D M, Holbeck S, Seyfried C, Byers P, Palmer J, Nepom G T
Virginia Mason Research Center, Seattle, WA 98101.
Genet Epidemiol. 1989;6(1):27-30. doi: 10.1002/gepi.1370060107.
A DQ beta locus-specific oligonucleotide probe is used to identify DQ beta alleles present in six insulin-dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer. Allele-specific oligonucleotide probes for the DR beta locus are used to identify subtypes (Dw4 and Dw14) of the DR4 specificity in five IDDM multiplex families. This distinction cannot be made with available cDNA probes.
一种DQβ基因座特异性寡核苷酸探针被用于识别六个胰岛素依赖型糖尿病（IDDM）多发家系中存在的DQβ等位基因。使用该技术进行基因分型的结果与使用cDNA探针得到的结果一致，而且往往更清晰。DRβ基因座的等位基因特异性寡核苷酸探针被用于识别五个IDDM多发家系中DR4特异性的亚型（Dw4和Dw14）。使用现有的cDNA探针无法做出这种区分。
